Design and Synthesis of an Activatable Photoacoustic Probe for Hypochlorous Acid

Takayuki Ikeno, Kenjiro Hanaoka, Shimpei Iwaki, Takuya Myochin, Yoshiaki Murayama, Hisashi Ohde, Toru Komatsu, Tasuku Ueno, Tetsuo Nagano, Yasuteru Urano

研究成果: Article査読

15 被引用数 (Scopus)

抄録

Photoacoustic (PA) imaging is a novel imaging modality that combines the high contrast of optical imaging and the deep tissue penetration of ultrasound. PA imaging contrast agents targeting various biological phenomena have been reported, but the development of activatable PA probes, which show a PA signal only in the presence of target molecules, remains challenging in spite of their potential usefulness for real-time PA imaging of specific biomolecules in vivo. To establish a simple design strategy for activatable PA probes, we first designed and synthesized a silicon-rhodamine based near-infrared nonfluorescent dye, wsSiNQ660 (water-soluble SiNQ660), as a scaffold and demonstrated that it offers a high conversion efficiency from light to ultrasound compared to typical near-infrared fluorescent dyes. Importantly, absorption off/on strategies previously established for rhodamine-based fluorescent probes are also applicable to this nonfluorescent dye scaffold. We validated this approach by synthesizing an activatable PA probe for hypochlorous acid (HOCl) and confirmed that it enables three-dimensional imaging of HOCl in mouse subcutis.

本文言語English
ページ(範囲)9086-9092
ページ数7
ジャーナルAnalytical Chemistry
91
14
DOI
出版ステータスPublished - 2019 6 20
外部発表はい

ASJC Scopus subject areas

  • 分析化学

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