Design and synthesis of photocleavable biotinylated-dopamine with polyethyleneoxy photocleavable linkers

Kengo Hanaya, Yoshiyuki Kageyama, Masanori Kitamura, Shin Aoki

研究成果: Article査読

4 被引用数 (Scopus)

抄録

Previously, we reported the synthesis of a biotin-dopamine conjugate (Btn-DA) with a photocleavable 8-quinolinyl benzenesulfonate (QB) linker for the isolation of an intact complex of DA and anti-DA antibody (IgG1) (DA-IgG1 complex). In this work, we synthesized a photocleavable Btn-DA conjugate with a polyethyleneoxy linker to improve the complexation efficiency and the recovery yields of DA-IgG1 complex. The results of QCM, ELISA, and Western blot analyses indicate that the introduction of polyethyleneoxy linkers improved the efficiency of DA-IgG1 complexation.

本文言語English
ページ(範囲)1601-1615
ページ数15
ジャーナルHeterocycles
82
2
DOI
出版ステータスPublished - 2010 2 28
外部発表はい

ASJC Scopus subject areas

  • 分析化学
  • 薬理学
  • 有機化学

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