Designed horizontal transfer of stable giant DNA released from Escherichia coli

Shinya Kaneko, Mitsuhiro Itaya

研究成果: Article

12 引用 (Scopus)

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DNA in the environment is a source to mediate horizontal gene transfer (HGT). Present molecular cloning methods are based on this HGT principle. However, DNA in the extracellular environment, particularly with high molecular-weight, is thought to be prone to shearing or digestion by nucleases. Here we discovered that extracellular plasmid DNA released from lysed Escherichia coli remained intact and stable. Furthermore, it was demonstrated that plasmids up to 100 kb in size were taken up by co-present competent Bacillus subtilis cells. The detailed kinetics of the process together with sensitivity to added DNase I indicated that plasmid DNA released from lysed E. coli into the culture medium was stable enough for quantitative efficacy in the transformation of B. subtilis. Our results will be useful for the development of methods to transfer giant DNAs from general host E. coli without their biochemical purification.

元の言語English
ページ(範囲)819-822
ページ数4
ジャーナルJournal of biochemistry
147
発行部数6
DOI
出版物ステータスPublished - 2010 6 1

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

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