Designed horizontal transfer of stable giant DNA released from Escherichia coli

Shinya Kaneko, Mitsuhiro Itaya

研究成果: Article査読

12 被引用数 (Scopus)

抄録

DNA in the environment is a source to mediate horizontal gene transfer (HGT). Present molecular cloning methods are based on this HGT principle. However, DNA in the extracellular environment, particularly with high molecular-weight, is thought to be prone to shearing or digestion by nucleases. Here we discovered that extracellular plasmid DNA released from lysed Escherichia coli remained intact and stable. Furthermore, it was demonstrated that plasmids up to 100 kb in size were taken up by co-present competent Bacillus subtilis cells. The detailed kinetics of the process together with sensitivity to added DNase I indicated that plasmid DNA released from lysed E. coli into the culture medium was stable enough for quantitative efficacy in the transformation of B. subtilis. Our results will be useful for the development of methods to transfer giant DNAs from general host E. coli without their biochemical purification.

本文言語English
ページ(範囲)819-822
ページ数4
ジャーナルJournal of biochemistry
147
6
DOI
出版ステータスPublished - 2010 6月

ASJC Scopus subject areas

  • 生化学
  • 分子生物学

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