Detection and characterization of DNA polymerase activity in Entamoeba histolytica

A. Makioka, S. Kobayashi, T. Takeuchi

研究成果: Article査読

10 被引用数 (Scopus)

抄録

DNA polymerase activity was detected and characterized in nuclear extracts from trophozoites of Entamoeba histolytica. The activity was high at pH 2 to pH 6, but at pH 8 and 10 the activity was very low. The presence of K+ was inhibitory for the activity and a higher concentration of K+ markedly inhibited the activity. Magnesium ions (Mg2+) were absolutely required for activity and its optimal concentration was 6 to 8 mM. The activity was markedly inhibited by aphidicolin which is an inhibitor of mammalian DNA polymerases α, δ, and ε and also by N-ethylmaleimide which is an inhibitor of DNA polymerases, α, γ, δ and ε. However, inhibition of the activity by 2',3'-dideoxythymidine-5'-triphosphate which is an inhibitor of DNA polymerases β and γ was relatively weak. Thus sensitivity of the E. histolytica enzyme to these inhibitors was similar to that of mammalian DNA polymerases (α, δ and ε) of the α family. Monoclonal antibodies against human DNA polymerase α did not bind to DNA polymerase of E. histolytica.

本文言語English
ページ(範囲)87-89
ページ数3
ジャーナルParasitology Research
82
1
DOI
出版ステータスPublished - 1996
外部発表はい

ASJC Scopus subject areas

  • 寄生虫科
  • 獣医学(全般)
  • 昆虫科学
  • 感染症

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