TY - JOUR
T1 - Detection and characterization of endothelin-1-like immunoreactivity in rat plasma
AU - Saito, Yoshihiko
AU - Nakao, Kazuwa
AU - Shirakami, Gotaro
AU - Jougasaki, Michihisa
AU - Yamada, Takayuki
AU - Itoh, Hiroshi
AU - Mukoyama, Masashi
AU - Arai, Hiroshi
AU - Hosoda, Kiminori
AU - Suga, Shin ichi
AU - Ogawa, Yoshihiro
AU - Imura, Hiroo
N1 - Funding Information:
We thank Ms. H. Fumon, Ms. A. Furu and N. Sako for their secretarial assistance and Ms. S. Morichika for her technical assistance. This work was supported in part by research grants from the Japanese Ministry of Education, Science and Culture, the Japanese Ministry of Health and Welfare "Disorders of Adrenal Hormone" Research Committee, Japan, 1989, Life Science Research Project of Institute of Physical and Chemical Research (RIKEN), Japan Tobacco Inc. and Yamanouchi Foundation for Research on Metabolic Disorders, by research grants for cardiovascular diseases (62A'-1 and 636-2) from the Japanese Ministry of Health and Welfare.
PY - 1989/9/29
Y1 - 1989/9/29
N2 - Using two radioimmunoassays (RIAs) for endothelin-1 (ET-1) with and without a substantial cross-reactivity with ET-3, we have measured the plasma ET-1-like immunoreactivity (-LI) level in rat plasma. ET-1-LI was detected in plasma from male Wistar rats. ET-1-LI in rat plasma consisted of three components with molecular weights of 6K, 4K and 2.5K daltons by gel permeation chromatography. Two of the components were eluted at positions of big ET (4K) and synthetic ET-1 (2.5K). The remaining component was eluted at the preceding fraction (6K). No difference was observed in ET-1-LI of the small molecular form of ET (2.5K) between the two RIAs. Thus, there is little or no ET-3 in rat plasma, which has the sequence found originally in the rat genome. The concentration of the small molecular form of ET, presumably ET-1, in rat plasma was about 4 pg/ml.
AB - Using two radioimmunoassays (RIAs) for endothelin-1 (ET-1) with and without a substantial cross-reactivity with ET-3, we have measured the plasma ET-1-like immunoreactivity (-LI) level in rat plasma. ET-1-LI was detected in plasma from male Wistar rats. ET-1-LI in rat plasma consisted of three components with molecular weights of 6K, 4K and 2.5K daltons by gel permeation chromatography. Two of the components were eluted at positions of big ET (4K) and synthetic ET-1 (2.5K). The remaining component was eluted at the preceding fraction (6K). No difference was observed in ET-1-LI of the small molecular form of ET (2.5K) between the two RIAs. Thus, there is little or no ET-3 in rat plasma, which has the sequence found originally in the rat genome. The concentration of the small molecular form of ET, presumably ET-1, in rat plasma was about 4 pg/ml.
UR - http://www.scopus.com/inward/record.url?scp=0024473904&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0024473904&partnerID=8YFLogxK
U2 - 10.1016/0006-291X(89)91151-0
DO - 10.1016/0006-291X(89)91151-0
M3 - Article
C2 - 2675844
AN - SCOPUS:0024473904
SN - 0006-291X
VL - 163
SP - 1512
EP - 1516
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 3
ER -