Detection and characterization of endothelin-1-like immunoreactivity in rat plasma

Yoshihiko Saito, Kazuwa Nakao, Gotaro Shirakami, Michihisa Jougasaki, Takayuki Yamada, Hiroshi Itoh, Masashi Mukoyama, Hiroshi Arai, Kiminori Hosoda, Shin ichi Suga, Yoshihiro Ogawa, Hiroo Imura

研究成果: Article査読

42 被引用数 (Scopus)


Using two radioimmunoassays (RIAs) for endothelin-1 (ET-1) with and without a substantial cross-reactivity with ET-3, we have measured the plasma ET-1-like immunoreactivity (-LI) level in rat plasma. ET-1-LI was detected in plasma from male Wistar rats. ET-1-LI in rat plasma consisted of three components with molecular weights of 6K, 4K and 2.5K daltons by gel permeation chromatography. Two of the components were eluted at positions of big ET (4K) and synthetic ET-1 (2.5K). The remaining component was eluted at the preceding fraction (6K). No difference was observed in ET-1-LI of the small molecular form of ET (2.5K) between the two RIAs. Thus, there is little or no ET-3 in rat plasma, which has the sequence found originally in the rat genome. The concentration of the small molecular form of ET, presumably ET-1, in rat plasma was about 4 pg/ml.

ジャーナルBiochemical and Biophysical Research Communications
出版ステータスPublished - 1989 9月 29

ASJC Scopus subject areas

  • 生物理学
  • 生化学
  • 分子生物学
  • 細胞生物学


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