TY - JOUR
T1 - Detection of a High-Turnover Serotonin Circuit in the Mouse Brain Using Mass Spectrometry Imaging
AU - Sugiyama, Eiji
AU - Guerrini, Matteo M.
AU - Honda, Kurara
AU - Hattori, Yuko
AU - Abe, Manabu
AU - Källback, Patrik
AU - Andrén, Per E.
AU - Tanaka, Kenji F.
AU - Setou, Mitsutoshi
AU - Fagarasan, Sidonia
AU - Suematsu, Makoto
AU - Sugiura, Yuki
PY - 2019/10/25
Y1 - 2019/10/25
N2 - Monoamine neurotransmitters are released by specialized neurons regulating behavioral, motor, and cognitive functions. Although the localization of monoaminergic neurons in the brain is well known, the distribution and kinetics of monoamines remain unclear. Here, we generated a murine brain atlas of serotonin (5-HT), dopamine (DA), and norepinephrine (NE) levels using mass spectrometry imaging (MSI). We found several nuclei rich in both 5-HT and a catecholamine (DA or NE) and identified the paraventricular nucleus of the thalamus (PVT), where 5-HT and NE are co-localized. The analysis of 5-HT fluctuations in response to acute tryptophan depletion and infusion of isotope-labeled tryptophan in vivo revealed a close kinetic association between the raphe nuclei, PVT, and amygdala but not the other nuclei. Our findings imply the existence of a highly dynamic 5-HT-mediated raphe to PVT pathway that likely plays a role in the brain monoamine system.
AB - Monoamine neurotransmitters are released by specialized neurons regulating behavioral, motor, and cognitive functions. Although the localization of monoaminergic neurons in the brain is well known, the distribution and kinetics of monoamines remain unclear. Here, we generated a murine brain atlas of serotonin (5-HT), dopamine (DA), and norepinephrine (NE) levels using mass spectrometry imaging (MSI). We found several nuclei rich in both 5-HT and a catecholamine (DA or NE) and identified the paraventricular nucleus of the thalamus (PVT), where 5-HT and NE are co-localized. The analysis of 5-HT fluctuations in response to acute tryptophan depletion and infusion of isotope-labeled tryptophan in vivo revealed a close kinetic association between the raphe nuclei, PVT, and amygdala but not the other nuclei. Our findings imply the existence of a highly dynamic 5-HT-mediated raphe to PVT pathway that likely plays a role in the brain monoamine system.
KW - Neuroscience
KW - Spectroscopy
KW - Systems Neuroscience
UR - http://www.scopus.com/inward/record.url?scp=85073028498&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85073028498&partnerID=8YFLogxK
U2 - 10.1016/j.isci.2019.09.036
DO - 10.1016/j.isci.2019.09.036
M3 - Article
AN - SCOPUS:85073028498
VL - 20
SP - 359
EP - 372
JO - iScience
JF - iScience
SN - 2589-0042
ER -