@article{ab4ccbb9bc3d406ca678bf52390fd274,
title = "Detection of a High-Turnover Serotonin Circuit in the Mouse Brain Using Mass Spectrometry Imaging",
abstract = "Monoamine neurotransmitters are released by specialized neurons regulating behavioral, motor, and cognitive functions. Although the localization of monoaminergic neurons in the brain is well known, the distribution and kinetics of monoamines remain unclear. Here, we generated a murine brain atlas of serotonin (5-HT), dopamine (DA), and norepinephrine (NE) levels using mass spectrometry imaging (MSI). We found several nuclei rich in both 5-HT and a catecholamine (DA or NE) and identified the paraventricular nucleus of the thalamus (PVT), where 5-HT and NE are co-localized. The analysis of 5-HT fluctuations in response to acute tryptophan depletion and infusion of isotope-labeled tryptophan in vivo revealed a close kinetic association between the raphe nuclei, PVT, and amygdala but not the other nuclei. Our findings imply the existence of a highly dynamic 5-HT-mediated raphe to PVT pathway that likely plays a role in the brain monoamine system.",
keywords = "Neuroscience, Spectroscopy, Systems Neuroscience",
author = "Eiji Sugiyama and Guerrini, {Matteo M.} and Kurara Honda and Yuko Hattori and Manabu Abe and Patrik K{\"a}llback and Andr{\'e}n, {Per E.} and Tanaka, {Kenji F.} and Mitsutoshi Setou and Sidonia Fagarasan and Makoto Suematsu and Yuki Sugiura",
note = "Funding Information: This work was supported by JSPS KAKENHI under grant number 16H06145 (to Y.S.); AMED under grant numbers JP18gm0710012 (to S.F. and Y.S.), JP18dm030727 (to Y.S. and E.S.), and JP18gm0910004 (to M.S.); AMED– CREST under grant number 14532135 (to S.F.); the Takeda Science Foundation (to E.S.); the Swedish Research Council (Medicine and Health no. 2018–03320, Natural and Engineering Science no. 2018–05501); the Swedish Brain Foundation ; the Swedish Foundation for Strategic (no. RIF14-0078 ); and Science for Life Laboratory (SciLifeLab) (to P.E.A). Publisher Copyright: {\textcopyright} 2019 The Author(s)",
year = "2019",
month = oct,
day = "25",
doi = "10.1016/j.isci.2019.09.036",
language = "English",
volume = "20",
pages = "359--372",
journal = "iScience",
issn = "2589-0042",
publisher = "Elsevier Inc.",
}