Detection of autoantibodies against bullous pemphigoid and pemphigus antigens by an enzyme‐linked immunosorbent assay using the bacterial recombinant proteins

Akiko Ide, Takashi Hashimoto, Masayuki Amagai, Masaru Tanaka, Takeji Nishikawa

研究成果: Article査読

28 被引用数 (Scopus)

抄録

Abstract To develop a new method to evaluate autoantibodies in various autoimmune bullous skin diseases, we examined reactivity of bullous pemphigoid (BP) and pemphigus vulgaris (PV) patients' sera with partial bacterial fusion proteins of the 230 kD BP antigen (BPAG1) and PV antigen, respectively, by an enzyme‐linked immunosorbent assay (ELISA), and compared the results with those of immunoblotting. We used two fusion proteins derived from the mouse BPAG1 and a fusion protein derived from the amino‐terminus (EC 1‐2) of PV antigen. For both BP and PV sera, the ELISA scores were well correlated with the reactivities on immunoblot assays. The present study indicates that ELISA using recombinant antigen proteins in various autoimmune bullous skin diseases will be a new useful technique to detect the autoantibodies. However, development of recombinant proteins with the entire molecule and correct conformation will be necessary to establish a perfect ELISA system in the future.

本文言語English
ページ(範囲)112-116
ページ数5
ジャーナルExperimental Dermatology
4
2
DOI
出版ステータスPublished - 1995 4

ASJC Scopus subject areas

  • 生化学
  • 分子生物学
  • 皮膚病学

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