抄録
Promoter activities of the brain-specific genes for glial fibrillary acidic protein (GFAP) and myelin basic (MBP) were investigated in brain cells in primary culture with the use of a novel retrovirus vector, pIP200. With this vector, promoter activity can be expressed in terms of β-galactosidase activity. Differentiation of the primary brain cells to mature glial cells was not affected by treatment with the pIP200 virus vector. The 256-bp 5'-flanking region of the GFAP gene directed astrocyte-specific expression of lacZ. It was silent in fibroblasts, even in multiple copies. The 1.3-kb 5'-flanking region of the MBP gene exhibited strict tissue (oligodendrocyte) specificity under the present assay method but showed some leakiness when integrated into the chromosome in multiple copies. Promoter regions conferring cell type specificity in brain were effectively identified by the present method.
| 元の言語 | English |
|---|---|
| ページ（範囲） | 53-60 |
| ページ数 | 8 |
| ジャーナル | New Biologist |
| 巻 | 4 |
| 発行部数 | 1 |
| 出版物ステータス | Published - 1992 |
| 外部発表 | Yes |
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ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)
これを引用
Detection of brain-specific gene expression in brain cells in primary culture : A novel promoter assay based on the use of a retrovirus vector. / Ikenaka, K.; Nakahira, K.; Nakajima, Kazunori; Fujimoto, I.; Kagawa, T.; Ogawa, M.; Mikoshiba, K.
：: New Biologist, 巻 4, 番号 1, 1992, p. 53-60.研究成果: Article
}
TY - JOUR
T1 - Detection of brain-specific gene expression in brain cells in primary culture
T2 - A novel promoter assay based on the use of a retrovirus vector
AU - Ikenaka, K.
AU - Nakahira, K.
AU - Nakajima, Kazunori
AU - Fujimoto, I.
AU - Kagawa, T.
AU - Ogawa, M.
AU - Mikoshiba, K.
PY - 1992
Y1 - 1992
N2 - Promoter activities of the brain-specific genes for glial fibrillary acidic protein (GFAP) and myelin basic (MBP) were investigated in brain cells in primary culture with the use of a novel retrovirus vector, pIP200. With this vector, promoter activity can be expressed in terms of β-galactosidase activity. Differentiation of the primary brain cells to mature glial cells was not affected by treatment with the pIP200 virus vector. The 256-bp 5'-flanking region of the GFAP gene directed astrocyte-specific expression of lacZ. It was silent in fibroblasts, even in multiple copies. The 1.3-kb 5'-flanking region of the MBP gene exhibited strict tissue (oligodendrocyte) specificity under the present assay method but showed some leakiness when integrated into the chromosome in multiple copies. Promoter regions conferring cell type specificity in brain were effectively identified by the present method.
AB - Promoter activities of the brain-specific genes for glial fibrillary acidic protein (GFAP) and myelin basic (MBP) were investigated in brain cells in primary culture with the use of a novel retrovirus vector, pIP200. With this vector, promoter activity can be expressed in terms of β-galactosidase activity. Differentiation of the primary brain cells to mature glial cells was not affected by treatment with the pIP200 virus vector. The 256-bp 5'-flanking region of the GFAP gene directed astrocyte-specific expression of lacZ. It was silent in fibroblasts, even in multiple copies. The 1.3-kb 5'-flanking region of the MBP gene exhibited strict tissue (oligodendrocyte) specificity under the present assay method but showed some leakiness when integrated into the chromosome in multiple copies. Promoter regions conferring cell type specificity in brain were effectively identified by the present method.
UR - http://www.scopus.com/inward/record.url?scp=0026574077&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0026574077&partnerID=8YFLogxK
M3 - Article
C2 - 1536830
AN - SCOPUS:0026574077
VL - 4
SP - 53
EP - 60
JO - New Biologist
JF - New Biologist
SN - 1043-4674
IS - 1
ER -