Detection of cRNA hybridized on a DNA chip using a tetrakis-acridinyl peptide cassette, consisting of TAP and partially self-complementary oligonucleotide, d[A 18 (TA) 51 ]

Katsuhiro Kawaai, Yasumitsu Kondoh, Takahiko Nojima, Kazuoki Tada, Shigeori Takenaka, Hideo Tashiro, Tomoko Tashiro

研究成果: Article査読

1 被引用数 (Scopus)

抄録

A tetrakis-acridinyl peptide (TAP) cassette, consisting of a double-stranded region of alternating AT sequence bound to TAP and a single stranded overhanging sequence of continuous dA, was prepared by mixing TAP with d[A 18 (TA) 51 ]. A TAP cassette could be applied to the fluorometric detection of hybridized DNA on the DNA chip, which was prepared by stamping a 45-meric DNA probe onto a gold-coated plastic chip using a high-precision spotter developed at RIKEN. Spots on the DNA chip were imaged by a CCD camera after hybridization with 65-meric target single-stranded DNAs carrying a continuous dA 20 sequence (dA tail) on the DNA chip after treatment with a TAP cassette. Their fluorescence intensity on the DNA chip showed a good linear correlation with the concentration of the target DNAs in the range from 10 pM to 1 nM. Fluorescence of their spots derived from the TAP cassette remaining on the surface of the DNA chip through the dA tail of the hybridized target DNA. Furthermore, the TAP cassette could be successfully applied to the quantitative detection of complementary RNAs (cRNAs) prepared from rat brain with reverse transcription and in vitro transcription. 2007

本文言語English
ページ(範囲)267-270
ページ数4
ジャーナルanalytical sciences
23
3
DOI
出版ステータスPublished - 2007 3
外部発表はい

ASJC Scopus subject areas

  • 分析化学

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「Detection of cRNA hybridized on a DNA chip using a tetrakis-acridinyl peptide cassette, consisting of TAP and partially self-complementary oligonucleotide, d[A <sub>18</sub> (TA) <sub>51</sub> ]」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

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