Detection of genes expressed in primary colon cancers by in situ hybridisation: Overexpression of RACK 1

A. Saito, G. Fujii, Y. Sato, M. Gotoh, Michiie Sakamoto, G. Toda, S. Hirohashi

研究成果: Article

32 引用 (Scopus)

抄録

Aims: The isolation of various genes that are expressed in a region specific manner is considered useful for research in molecular pathology. In situ hybridisation (ISH) was used in a screening procedure to isolate these genes efficiently, using colon cancer as a model. Methods: Suppression subtractive hybridisation (SSH) between colon cancer tissue samples and corresponding non-cancerous tissues was performed. Genes showing high expression in the cancers were selected using macro-DNA array analysis. As a final screening procedure, conventional ISH was performed to isolate genes expressed specifically in colon cancers. Results: Sixty nine clones were selected by SSH and macro-DNA array analyses. These clones were then analysed by ISH to examine their expression patterns. ISH screening revealed that all the clones screened showed more intense signals in colon cancers than in non-cancerous tissues. Among them, RACK 1, which is a protein kinase C receptor and a homologue of the G protein β subunit, was expressed intensely in colon cancer cells. RACK 1 expression was evaluated in multiple samples by ISH, and the results confirmed that RACK 1 was universally overexpressed in cells of all 11 colon cancers examined. Conclusions: Many genes, including RACK 1, expressed in colon cancer cells can be isolated efficiently by this method, and their precise expression pattern can be evaluated. These results indicate that ISH is an excellent technique for systemic screening of genes expressed in a region specific manner.

元の言語English
ページ(範囲)34-39
ページ数6
ジャーナルJournal of Clinical Pathology - Molecular Pathology
55
発行部数1
DOI
出版物ステータスPublished - 2002
外部発表Yes

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Colonic Neoplasms
In Situ Hybridization
Genes
Clone Cells
Oligonucleotide Array Sequence Analysis
Molecular Pathology
Protein Subunits
GTP-Binding Proteins
Research
Neoplasms

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

これを引用

Detection of genes expressed in primary colon cancers by in situ hybridisation : Overexpression of RACK 1. / Saito, A.; Fujii, G.; Sato, Y.; Gotoh, M.; Sakamoto, Michiie; Toda, G.; Hirohashi, S.

:: Journal of Clinical Pathology - Molecular Pathology, 巻 55, 番号 1, 2002, p. 34-39.

研究成果: Article

Saito, A. ; Fujii, G. ; Sato, Y. ; Gotoh, M. ; Sakamoto, Michiie ; Toda, G. ; Hirohashi, S. / Detection of genes expressed in primary colon cancers by in situ hybridisation : Overexpression of RACK 1. :: Journal of Clinical Pathology - Molecular Pathology. 2002 ; 巻 55, 番号 1. pp. 34-39.
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AU - Sakamoto, Michiie

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AU - Hirohashi, S.

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