Detection of IgA autoantibodies to desmogleins by an enzyme-linked immunosorbent assay: The presence of new minor subtypes of IgA pemphigus

Takashi Hashimoto, Ayako Komai, Yuko Futei, Takeji Nishikawa, Masayuki Amagai

研究成果: Article査読

73 被引用数 (Scopus)

抄録

Objective: To examine the frequency of antidesmoglein 1 (Dsg1) and antidesmoglein 3 (Dsg3) IgA autoantibodies in IgA pemphigus. Design: We developed an enzyme-linked immunosorbent assay against recombinant Dsg1 and Dsg3 to detect IgA autoantibodies. Patients: Twenty-two patients with IgA pemphigus were studied. Among them, 10 patients had subcorneal pustular dermatosis type, 9 patients had intraepidermal neutrophilic IgA dermatosis type, and 3 patients had pemphigus foliaceus-like clinical features. Results: Of the 22 cases of IgA pemphigus, 3 cases were positive for anti-Dsg1 IgA antibodies and only 1 case was positive for anti-Dsg3 IgA antibodies. In those 4 cases, there were no IgA autoantibodies against other components of the keratinocyte cell surfaces because preincubation with the respective recombinant desmogleins removed the immunoreactivity on immunofluorescence. All 10 patients with subcorneal pustular dermatosis type IgA pemphigus were positive against desmocollin 1 expressed on COS-7 cells. No target antigen was detected in the other 8 cases. Conclusions: Desmogleins were recognized by IgA antibodies of a few patients with IgA pemphigus. Considering that subcorneal pustular dermatosis type IgA pemphigus recognizes desmocollin 1, autoimmune targets of IgA pemphigus are more heterogeneous than previously considered.

本文言語English
ページ(範囲)735-738
ページ数4
ジャーナルArchives of Dermatology
137
6
出版ステータスPublished - 2001

ASJC Scopus subject areas

  • 皮膚病学

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