Detection of Influenza Virus by Agglutination of Microparticles Immobilized a Mixed Glycan Receptor Produced from Cells

Teruhiko Matsubara, Ayaka Ogami, Haruka Kori, Mineo Hashizume, Toshinori Sato

研究成果: Article査読

抄録

The hemagglutination inhibition (HAI) assay is one of the detection methods for influenza virus (IFV) under global influenza surveillance, which uses freshly prepared animal red blood cells (RBCs). Here, we demonstrate that a mixed glycan-modified polystyrene microparticle, which can be chemically prepared in advance, can replace animal RBCs in the HAI assay. A mixture of azide-conjugated glycans containing sialyl- and sulfated-lactose moieties was produced from Madin-Darby canine kidney (MDCK) cells, which are used for IFV isolation, and then immobilized on the surface of a polystyrene microparticle using click chemistry. Human HA and IFV were detected with high sensitivity when using the mixed glycan-immobilized particle.

本文言語English
ジャーナルACS Applied Bio Materials
DOI
出版ステータスAccepted/In press - 2022

ASJC Scopus subject areas

  • 生体材料
  • 化学 (全般)
  • 生体医工学
  • 生化学、医学

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