Detection of pathogenic entamoeba histolytica DNA in liver abscess fluid by polymerase chain reaction

H. Tachibana, S. Kobayashi, E. Okuzawa, G. Masuda

研究成果: Article査読

36 被引用数 (Scopus)

抄録

Tachibana H., Kobayashi S., Okuzawa E. and Masuda G. 1992. Detection of pathogenic Entamoeba histolytica DNA in liver abscess fluid by polymerase chain reaction. International Journal for Parasitology 22: 1193-1196. A sensitive method for detection of pathogenic Entamoeba histolytica DNA in drained fluids from liver abscess patients, using the polymerase chain reaction (PCR), has been developed. The PCR employs oligonucleotide primers specific for the gene encoding the 30 kDa molecule of pathogenic E. histolytica. Liver abscess fluids (19 samples), from 14 patients with a presumptive amebic liver abscess, were examined microscopically and by the PCR method. Only two of the 19 samples were positive microscopically, whereas all 19 samples tested positive by PCR. This technique can be used to confirm the diagnosis of amebic liver abscess.

本文言語English
ページ(範囲)1193-1196
ページ数4
ジャーナルInternational Journal for Parasitology
22
8
DOI
出版ステータスPublished - 1992 12月
外部発表はい

ASJC Scopus subject areas

  • 寄生虫科
  • 感染症

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