Detection of Proteins that Recognize Platinum‐modified DNA Using Gel Mobility Shift Assay

Yasuhiro Fujiwara, Kazuo Kasahara, Yoshikazu Sugimoto, Kazuto Nishio, Tohru Ohmori, Nagahiro Saijo

研究成果: Article査読

7 被引用数 (Scopus)

抄録

Using a gel mobility shift assay, we have identified proteins, in the nuclear extracts of a human lung cancer cell line, that recognize cis‐diamminedichloroplatinum(II) (cis‐DDP, CDDP)‐modified DNA. A 158‐base‐pair double‐stranded DNA fragment, derived from pBR322 plasmid DNA, was modified by either CDDP, tetrachloro(dl‐trans)‐1,2‐diaminocyclohexaneplatinum(IV) (tetraplatin) or trans‐DDP (the stereoisomer of CDDP and clinically ineffective). These platinum drug‐modified probes were incubated with nuclear extracts and analyzed by gel mobility shift assay. Proteins in the extracts selectively recognized the clinically active platinum‐modified DNA fragment. No binding to the trans‐DDP‐modified DNA fragment was observed. These proteins may play a role in the cytotoxicity or in a DNA repair process.

本文言語English
ページ(範囲)1210-1213
ページ数4
ジャーナルJapanese Journal of Cancer Research
81
12
DOI
出版ステータスPublished - 1990 12月
外部発表はい

ASJC Scopus subject areas

  • 腫瘍学
  • 癌研究

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