Detection of soluble forms of platelet membrane glycoproteins and its clinical significance

M. Handa, M. Katayama, Y. Ikeda

研究成果: Article査読

2 被引用数 (Scopus)

抄録

Soluble forms of surface membrane glycoproteins of various cell types have been identified as makers for in vivo or in vitro cellular events, in which the receptors are involved. Using mouse monoclonal antibodies, sandwich ELISA systems to quantitate plasma concentrations of two platelet glycoproteins in soluble form were set up: one is glycocalicin (GC), a proteolytic fragment of GP Ib alpha chain and the other is GMP-140 or CD62 (GMP) known as a target for leukocyte recruitment at vascular injury sites. As in vivo markers, GC is said to reflect platelet turnover. High levels of plasma GMP were detected in patients with thrombotic thrombocytopenic purpura, being presumably indicative of microcirculation disorder. During storage of platelet concentrates, plasma levels of both markers were increased in a time-dependent fashion over 10 days. This correlated with a decrease in the surface expression of the glycoproteins on platelets, suggesting that the soluble glycoproteins entering the supernatants by proteolysis would be an useful indicator for in vitro alteration of stored platelets surface.

本文言語English
ページ(範囲)360-365
ページ数6
ジャーナルNippon rinsho. Japanese journal of clinical medicine
50
2
出版ステータスPublished - 1992 2月

ASJC Scopus subject areas

  • 医学(全般)

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