Detection of von Willebrand factor-cleaving protease (ADAMTS-13) in human platelets

Misako Suzuki, Mitsuru Murata, Yumiko Matsubara, Toshihiro Uchida, Hiroaki Ishihara, Toshiro Shibano, Shin Ichiro Ashida, Kenji Soejima, Yasunori Okada, Yasuo Ikeda

研究成果: Article査読

93 被引用数 (Scopus)

抄録

The hemostatic activity of von Willebrand factor (vWF) is strongly dependent on its multimeric structure, with the highest activity in 'unusually large' multimers secreted from endothelial cells. The multimeric structure is regulated by a plasma protease, vWF-cleaving protease (vWF-CP, or ADAMTS-13). ADAMTS-13 mRNA is variably expressed in liver and other tissues. Because 15-25% of circulating vWF is stored in platelets, the presence and function of ADAMTS-13 in platelets are important issues. Here we report ADAMTS-13 expression in human platelets. Western blot analysis and flow cytometric analysis on permeabilized platelets revealed the presence of ADAMTS-13 protein. Real-time PCR demonstrated that ADAMTS-13 mRNA is present in platelets of six healthy volunteers, with little quantitative difference. The presence of ADAMTS-13 in platelets may imply the functional role of this enzyme in the local regulation of platelet function at the site of vascular injury or thrombus formation, and provide a useful tool for the analysis of structure and function of this enzyme.

本文言語English
ページ(範囲)212-216
ページ数5
ジャーナルBiochemical and Biophysical Research Communications
313
1
DOI
出版ステータスPublished - 2004 1月 2
外部発表はい

ASJC Scopus subject areas

  • 生物理学
  • 生化学
  • 分子生物学
  • 細胞生物学

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