Determination of carbohydrates by hydrophilic interaction chromatography with pulsed amperometric detection using postcolumn pH adjustment

Tomoyoshi Soga, Yoshinori Inoue, Kenji Yamaguchi

研究成果: Article査読

29 被引用数 (Scopus)

抄録

An HPLC method for the determination of carbohydrates using a postcolumn pH adjustment technique with lithium hydroxide solution followed by pulsed amperometric detection was developed. In conjunction with hydrophilic interaction chromatography, the technique was used to determine reducing sugars, non-reducing sugars and sugar alcohols. Detection limits from 0.7 to 2.7 ng with a signal-to-noise ratio of 3 were achieved. The relative standard deviation for peak was better than 2.7%. The postcolumn pH adjustment technique enabled gradient elution to be used.

本文言語English
ページ(範囲)151-155
ページ数5
ジャーナルJournal of Chromatography A
625
2
DOI
出版ステータスPublished - 1992 11月 20
外部発表はい

ASJC Scopus subject areas

  • 分析化学
  • 生化学
  • 有機化学

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