Determination of copper(II) chlorophyllin by reversed-phase high-performance liquid chromatography

H. Inoue, H. Yamashita, K. Furuya, Y. Nonomura, N. Yoshioka, S. Lib

研究成果: Article査読

47 被引用数 (Scopus)

抄録

Copper(II) chlorophyllin, consisting of copper(II) pheophorbide a, copper(II) chlorin e6, copper(II) rhodin g7 and copper (II) chlorin e4, was prepared and separated by semi-preparative high-performance liquid chromatography (HPLC). The components of copper(II) chlorophyllin were determined on a reversed-phase Inertsil ODS-2 column using a mobile phase of methanol-water (97:3, v/v) containing 1% (v/v) of acetic acid. Linear calibration plots were obtained for copper(II) chlorophyllin in the concentration range of 0-30 μg cm-3 with photometric detection at 407 or 423 nm. The detection limits of copper(II) pheophorbide a, copper(II) chlorin e6, copper(II) rhodin g7 and copper(II) chlorin e4 were 3.5, 1.5, 3.3 and 1.4 ng cm-3 with relative standard deviations (n = 10) of 1.8, 1.6, 5.2 and 3.6%, respectively. The reversed-phase HPLC method proposed here was demonstrated to be useful for the determination of the components of sodium copper(II) chlorophyllin.

本文言語English
ページ(範囲)99-104
ページ数6
ジャーナルJournal of Chromatography A
679
1
DOI
出版ステータスPublished - 1994 9月 9

ASJC Scopus subject areas

  • 分析化学
  • 生化学
  • 有機化学

フィンガープリント

「Determination of copper(II) chlorophyllin by reversed-phase high-performance liquid chromatography」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル