Determination of theophylline and its metabolites in biological samples by liquid chromatography-mass spectrometry

Hideko Kanazawa, Ryoko Atsumi, Yoshikazu Matsushima, Junko Kizu

研究成果: Article査読

52 被引用数 (Scopus)

抄録

Liquid chromatography-mass spectrometry (LC-MS) is a powerful tool for analysis of drugs and their metabolites. We used a column-switching system in combination with atmospheric pressure chemical ionization LC-MS (LC-APCI-MS) for the determination of theophylline and its metabolites in biological samples. The separation was carried out on a reversed-phase column using methanol-20 mM ammonium acetate as a mobile phase at a flow-rate of 1 ml/min in 30 min. In the mass spectrum, the molecular ions of these drugs and metabolites were clearly observed as base peaks. This method is sufficiently sensitive and accurate for the pharmacokinetic studies of these drugs. Copyright (C) 2000 Elsevier Science B.V.

本文言語English
ページ(範囲)87-96
ページ数10
ジャーナルJournal of Chromatography A
870
1-2
DOI
出版ステータスPublished - 2000 2 18

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Organic Chemistry

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