TY - JOUR
T1 - Determination of tissue inhibitor of metalloproteinases-2 (TIMP-2) in experimental animals using monoclonal antibodies against TIMP-2-specific oligopeptides
AU - Fujimoto, Noboru
AU - Tokai, Hideaki
AU - Iwata, Kazushi
AU - Okada, Yasunori
AU - Hayakawa, Taro
PY - 1995/11/16
Y1 - 1995/11/16
N2 - We have developed a one-step sandwich enzyme immunoassay (EIA) using monoclonal antibodies against oligopeptides of the human tissue inhibitor of metalloproteinases-2 (TIMP-2) (Fujimoto et al. (1993) Clin. Chim. Acta 220, 31). The present studies further demonstrated that the antibodies cross-react with TIMP-2 species of experimental animals including mouse, rat, guinea pig and rabbit. The detection of the TIMP-2 species in our EIA system was verified using rat TIMP-2 and the EIA was subsequently used to measure the animal TIMP-2 in the sera. Using human TIMP-2 as a standard, TIMP-2 levels in the sera of mouse, rat, guinea pig and rabbit were approximately 80, 200, 270 and 25 ng/ml, respectively.
AB - We have developed a one-step sandwich enzyme immunoassay (EIA) using monoclonal antibodies against oligopeptides of the human tissue inhibitor of metalloproteinases-2 (TIMP-2) (Fujimoto et al. (1993) Clin. Chim. Acta 220, 31). The present studies further demonstrated that the antibodies cross-react with TIMP-2 species of experimental animals including mouse, rat, guinea pig and rabbit. The detection of the TIMP-2 species in our EIA system was verified using rat TIMP-2 and the EIA was subsequently used to measure the animal TIMP-2 in the sera. Using human TIMP-2 as a standard, TIMP-2 levels in the sera of mouse, rat, guinea pig and rabbit were approximately 80, 200, 270 and 25 ng/ml, respectively.
KW - Enzyme immunoassay
KW - Matrix metalloproteinase
KW - Monoclonal antibody
KW - Tissue inhibitor of metalloproteinases-2
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U2 - 10.1016/0022-1759(95)00164-6
DO - 10.1016/0022-1759(95)00164-6
M3 - Article
C2 - 7490456
AN - SCOPUS:0028800424
SN - 0022-1759
VL - 187
SP - 33
EP - 39
JO - Journal of Immunological Methods
JF - Journal of Immunological Methods
IS - 1
ER -