Development of μtas for high-throughput screening of nad(p)-dependent oxidoreductases

Shota Shimokihara, Shunya Shitara, Ryo Oyobiki, Takeshi Watanabe, Yasuaki Einaga, Yoshinori Matsumoto, Kei Fujiwara, Kenichi Horisawa, Nobuhide Doi

研究成果: Conference contribution

2 引用 (Scopus)

抜粋

Although oxidoreductases are widely used in many applications, such as biosensors and biofuel cells, improvements in the function of existing oxidoreductases or the discovery of novel oxidoreductases with greater activity is desired. To increase the activity of oxidoreductases by directed evolution, a powerful screening technique for oxidoreductases is required. In this study, we developed a microfluidic total analysis system for high-throughput screening of NAD(P)-dependent oxidoreductases. This system comprises three microfluidic devices: an encapsulating device, a NADH measuring device, and a magnetic beads sorting device. We demonstrated the utility of each part of the system.

元の言語English
ホスト出版物のタイトルMicroTAS 2015 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences
出版者Chemical and Biological Microsystems Society
ページ1302-1304
ページ数3
ISBN(電子版)9780979806483
出版物ステータスPublished - 2015
イベント19th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2015 - Gyeongju, Korea, Republic of
継続期間: 2015 10 252015 10 29

Other

Other19th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2015
Korea, Republic of
Gyeongju
期間15/10/2515/10/29

ASJC Scopus subject areas

  • Control and Systems Engineering

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  • これを引用

    Shimokihara, S., Shitara, S., Oyobiki, R., Watanabe, T., Einaga, Y., Matsumoto, Y., Fujiwara, K., Horisawa, K., & Doi, N. (2015). Development of μtas for high-throughput screening of nad(p)-dependent oxidoreductases. : MicroTAS 2015 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences (pp. 1302-1304). Chemical and Biological Microsystems Society.