Development of μtas for high-throughput screening of nad(p)-dependent oxidoreductases

Shota Shimokihara; Shunya Shitara; Ryo Oyobiki; Takeshi Watanabe; Yasuaki Einaga; Yoshinori Matsumoto; Kei Fujiwara; Kenichi Horisawa; Nobuhide Doi

Development of μtas for high-throughput screening of nad(p)-dependent oxidoreductases

Shota Shimokihara, Shunya Shitara, Ryo Oyobiki, Takeshi Watanabe, Yasuaki Einaga, Yoshinori Matsumoto, Kei Fujiwara, Kenichi Horisawa, Nobuhide Doi

研究成果: Conference contribution

2 被引用数 (Scopus)

抄録

Although oxidoreductases are widely used in many applications, such as biosensors and biofuel cells, improvements in the function of existing oxidoreductases or the discovery of novel oxidoreductases with greater activity is desired. To increase the activity of oxidoreductases by directed evolution, a powerful screening technique for oxidoreductases is required. In this study, we developed a microfluidic total analysis system for high-throughput screening of NAD(P)-dependent oxidoreductases. This system comprises three microfluidic devices: an encapsulating device, a NADH measuring device, and a magnetic beads sorting device. We demonstrated the utility of each part of the system.

本文言語	English
ホスト出版物のタイトル	MicroTAS 2015 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences
出版社	Chemical and Biological Microsystems Society
ページ	1302-1304
ページ数	3
ISBN（電子版）	9780979806483
出版ステータス	Published - 2015
イベント	19th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2015 - Gyeongju, Korea, Republic of 継続期間: 2015 10月 25 → 2015 10月 29

Other

Other	19th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2015
国/地域	Korea, Republic of
City	Gyeongju
Period	15/10/25 → 15/10/29

ASJC Scopus subject areas

制御およびシステム工学

他のファイルとリンク

引用スタイル

Shimokihara, S., Shitara, S., Oyobiki, R., Watanabe, T., Einaga, Y., Matsumoto, Y., Fujiwara, K., Horisawa, K., & Doi, N. (2015). Development of μtas for high-throughput screening of nad(p)-dependent oxidoreductases. In MicroTAS 2015 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences (pp. 1302-1304). Chemical and Biological Microsystems Society.

Shimokihara, S, Shitara, S, Oyobiki, R, Watanabe, T, Einaga, Y , Matsumoto, Y , Fujiwara, K, Horisawa, K & Doi, N 2015, Development of μtas for high-throughput screening of nad(p)-dependent oxidoreductases. in MicroTAS 2015 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences. Chemical and Biological Microsystems Society, pp. 1302-1304, 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2015, Gyeongju, Korea, Republic of, 15/10/25.

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abstract = "Although oxidoreductases are widely used in many applications, such as biosensors and biofuel cells, improvements in the function of existing oxidoreductases or the discovery of novel oxidoreductases with greater activity is desired. To increase the activity of oxidoreductases by directed evolution, a powerful screening technique for oxidoreductases is required. In this study, we developed a microfluidic total analysis system for high-throughput screening of NAD(P)-dependent oxidoreductases. This system comprises three microfluidic devices: an encapsulating device, a NADH measuring device, and a magnetic beads sorting device. We demonstrated the utility of each part of the system.",

keywords = "Biosensing, Directed evolution, High-throughput screening, Oxidoreductase",

author = "Shota Shimokihara and Shunya Shitara and Ryo Oyobiki and Takeshi Watanabe and Yasuaki Einaga and Yoshinori Matsumoto and Kei Fujiwara and Kenichi Horisawa and Nobuhide Doi",

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T1 - Development of μtas for high-throughput screening of nad(p)-dependent oxidoreductases

AU - Shimokihara, Shota

AU - Shitara, Shunya

AU - Oyobiki, Ryo

AU - Watanabe, Takeshi

AU - Einaga, Yasuaki

AU - Matsumoto, Yoshinori

AU - Fujiwara, Kei

AU - Horisawa, Kenichi

AU - Doi, Nobuhide

PY - 2015

Y1 - 2015

N2 - Although oxidoreductases are widely used in many applications, such as biosensors and biofuel cells, improvements in the function of existing oxidoreductases or the discovery of novel oxidoreductases with greater activity is desired. To increase the activity of oxidoreductases by directed evolution, a powerful screening technique for oxidoreductases is required. In this study, we developed a microfluidic total analysis system for high-throughput screening of NAD(P)-dependent oxidoreductases. This system comprises three microfluidic devices: an encapsulating device, a NADH measuring device, and a magnetic beads sorting device. We demonstrated the utility of each part of the system.

AB - Although oxidoreductases are widely used in many applications, such as biosensors and biofuel cells, improvements in the function of existing oxidoreductases or the discovery of novel oxidoreductases with greater activity is desired. To increase the activity of oxidoreductases by directed evolution, a powerful screening technique for oxidoreductases is required. In this study, we developed a microfluidic total analysis system for high-throughput screening of NAD(P)-dependent oxidoreductases. This system comprises three microfluidic devices: an encapsulating device, a NADH measuring device, and a magnetic beads sorting device. We demonstrated the utility of each part of the system.

KW - Biosensing

KW - Directed evolution

KW - High-throughput screening

KW - Oxidoreductase

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M3 - Conference contribution

AN - SCOPUS:84983317467

SP - 1302

EP - 1304

BT - MicroTAS 2015 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences

PB - Chemical and Biological Microsystems Society

T2 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2015

Y2 - 25 October 2015 through 29 October 2015

ER -

Development of μtas for high-throughput screening of nad(p)-dependent oxidoreductases

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Other

ASJC Scopus subject areas

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