Development of a functional thyroid model based on an organoid culture system

Yoshiyuki Saito, Nobuyuki Onishi, Hiroshi Takami, Ryo Seishima, Hiroyoshi Inoue, Yuki Hirata, Kaori Kameyama, Kenji Tsuchihashi, Eiji Sugihara, Shinya Uchino, Koichi Ito, Hirofumi Kawakubo, Hiroya Takeuchi, Yuko Kitagawa, Hideyuki Saya, Osamu Nagano

研究成果: Article査読

21 被引用数 (Scopus)

抄録

The low turnover rate of thyroid follicular cells and the lack of a long-term thyroid cell culture system have hampered studies of thyroid carcinogenesis. We have now established a thyroid organoid culture system that supports thyroid cell proliferation in vitro. The established mouse thyroid organoids performed thyroid functions including thyroglobulin synthesis, iodide uptake, and the production and release of thyroid hormone. Furthermore, transplantation of the organoids into recipient mice resulted in the formation of normal thyroid–like tissue capable of iodide uptake and thyroglobulin production in vivo. Finally, forced expression of oncogenic NRAS (NRASQ61R) in thyroid organoids established from p53 knockout mice and transplantation of the manipulated organoids into mouse recipients generated a model of poorly differentiated thyroid cancer. Our findings suggest that this newly developed thyroid organoid culture system is a potential research tool for the study of thyroid physiology and pathology including thyroid cancer.

本文言語English
ページ(範囲)783-789
ページ数7
ジャーナルBiochemical and Biophysical Research Communications
497
2
DOI
出版ステータスPublished - 2018 3 4

ASJC Scopus subject areas

  • 生物理学
  • 生化学
  • 分子生物学
  • 細胞生物学

フィンガープリント

「Development of a functional thyroid model based on an organoid culture system」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル