Development of a high-efficacy reprogramming method for generating human induced pluripotent stem (Ips) cells from pathologic and senescent somatic cells

Naomichi Tanaka, Hidemasa Kato, Hiromi Tsuda, Yasunori Sato, Toshihiro Muramatsu, Atsushi Iguchi, Hiroyuki Nakajima, Akihiro Yoshitake, Takaaki Senbonmatsu

研究成果: Article査読

抄録

Induced pluripotent stem (iPS) cells are a type of artificial pluripotent stem cell induced by the epigenetic silencing of somatic cells by the Yamanaka factors. Advances in iPS cell reprogramming technology will allow aging or damaged cells to be replaced by a patient’s own rejuvenated cells. However, tissue that is senescent or pathologic has a relatively low reprogramming efficiency as compared with juvenile or robust tissue, resulting in incomplete reprogramming; iPS cells generated from such tissue types do not have sufficient differentiation ability and are therefore difficult to apply clinically. Here, we develop a new reprogramming method and examine it using myofibroblasts, which are pathologic somatic cells, from patient skin tissue and from each of the four heart chambers of a recipient heart in heart transplant surgery. By adjusting the type and amount of vectors containing transcriptional factors for iPS cell reprogramming, as well as adjusting the transfection load and culture medium, the efficiency of iPS cell induction from aged patient skin-derived fibroblasts was increased, and we successfully induced iPS cells from myocardial fibroblasts isolated from the pathologic heart of a heart transplant recipient.

本文言語English
論文番号6764
ページ(範囲)1-16
ページ数16
ジャーナルInternational journal of molecular sciences
21
18
DOI
出版ステータスPublished - 2020 9 2

ASJC Scopus subject areas

  • Catalysis
  • Molecular Biology
  • Spectroscopy
  • Computer Science Applications
  • Physical and Theoretical Chemistry
  • Organic Chemistry
  • Inorganic Chemistry

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