TY - JOUR
T1 - Development of a new neutralization test for measles virus
AU - Fujino, Motoko
AU - Yoshida, Naoko
AU - Kimura, Keiko
AU - Zhou, Jianhui
AU - Motegi, Yoshie
AU - Komase, Katsuhiro
AU - Nakayama, Tetsuo
N1 - Funding Information:
This study was supported by a Grant from the 21st Century COE Program of the Ministry of Education, Culture, Sports, Science, and Technology of Japan.
PY - 2007/6
Y1 - 2007/6
N2 - Sero-epidemiological studies are required to identify populations susceptible to measles. The hemagglutination inhibition (HI) test is no longer sensitive enough to confirm immunity to measles, and at present the particle agglutination (PA) test and enzyme-linked immunosorbent assay (EIA) are employed. The most reliable method is the neutralization test (NT), but it is time-consuming and requires experience. To simplify the NT, a recombinant measles AIK-C virus expressing green fluorescence protein (GFP-MVAIK) was constructed and used as a challenge virus. Plaques and cytopathic effects were visualized under ultraviolet light and detected easily, and measuring the intensity of the fluorescence enabled a reduction in the time-consuming steps. Neutralizing antibody titers of a complete inhibition neutralization test were equivalent to those of a 90% plaque reduction neutralization test. Comparison of four methods, HI, PA, EIA and the complete inhibition neutralization test, showed that only the results of EIA correlated well with those of the complete inhibition neutralization test, but sera with borderline levels by EIA were sometimes negative by the complete inhibition neutralization assay.
AB - Sero-epidemiological studies are required to identify populations susceptible to measles. The hemagglutination inhibition (HI) test is no longer sensitive enough to confirm immunity to measles, and at present the particle agglutination (PA) test and enzyme-linked immunosorbent assay (EIA) are employed. The most reliable method is the neutralization test (NT), but it is time-consuming and requires experience. To simplify the NT, a recombinant measles AIK-C virus expressing green fluorescence protein (GFP-MVAIK) was constructed and used as a challenge virus. Plaques and cytopathic effects were visualized under ultraviolet light and detected easily, and measuring the intensity of the fluorescence enabled a reduction in the time-consuming steps. Neutralizing antibody titers of a complete inhibition neutralization test were equivalent to those of a 90% plaque reduction neutralization test. Comparison of four methods, HI, PA, EIA and the complete inhibition neutralization test, showed that only the results of EIA correlated well with those of the complete inhibition neutralization test, but sera with borderline levels by EIA were sometimes negative by the complete inhibition neutralization assay.
KW - Enzyme-linked immunosorbent assay (EIA)
KW - Hemagglutination inhibition (HI)
KW - Neutralization test (NT)
KW - Particle agglutination (PA)
KW - Recombinant measles virus
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U2 - 10.1016/j.jviromet.2007.01.001
DO - 10.1016/j.jviromet.2007.01.001
M3 - Article
C2 - 17320979
AN - SCOPUS:34247113710
SN - 0166-0934
VL - 142
SP - 15
EP - 20
JO - Journal of Virological Methods
JF - Journal of Virological Methods
IS - 1-2
ER -
