TY - JOUR
T1 - Development of a sensitive novel diagnostic kit for the highly pathogenic avian influenza A (H5N1) virus
AU - Tsunetsugu-Yokota, Yasuko
AU - Nishimura, Kengo
AU - Misawa, Shuhei
AU - Kobayashi-Ishihara, Mie
AU - Takahashi, Hitoshi
AU - Takayama, Ikuyo
AU - Ohnishi, Kazuo
AU - Itamura, Shigeyuki
AU - Nguyen, Hang L.K.
AU - Le, Mai T.Q.
AU - Dang, Giang T.
AU - Nguyen, Long T.
AU - Tashiro, Masato
AU - Kageyama, Tsutomu
N1 - Funding Information:
We thank all our colleagues in the First laboratory, and others in the Department of Immunology, NIID Japan, for their help and discussions. This work was supported by a grant from the Health Science Foundation (KHC1218) of the Ministry of Health, Labor and Welfare of Japan.
Funding Information:
KN and SM are research staffs in Toyobo Co.Ltd. and collaborated with YYY, KO and TK in NIID (a government research institute) for this study, which was formally supported by a grant from the Health Science Foundation of the Ministry of Health, Labor and Welfare of Japan (KHC1218). The patent of H5/A kit was filed by Toyobo Co. Ltd and NIID. This may not cause any competing non-financial interests were they to become public after the publication of this manuscript. All other authors declare no potential conflicts of interest.
PY - 2014/7/3
Y1 - 2014/7/3
N2 - Background: Sporadic emergence of the highly pathogenic avian influenza (HPAI) H5N1 virus infection in humans is a serious concern because of the potential for a pandemic. Conventional or quantitative RT-PCR is the standard laboratory test to detect viral influenza infections. However, this technology requires well-equipped laboratories and highly trained personnel. A rapid, sensitive, and specific alternative screening method is needed.Methods: By a luminescence-linked enzyme immunoassay, we have developed a H5N1 HPAI virus detection kit using anti-H5 hemagglutinin monoclonal antibodies in combination with the detection of a universal NP antigen of the type A influenza virus. The process takes 15 minutes by use of the fully automated luminescence analyzer, POCube.Resutls: We tested this H5/A kit using 19 clinical specimens from 13 patients stored in Vietnam who were infected with clade 1.1 or clade 2.3.4 H5N1 HPAI virus. Approximately 80% of clinical specimens were H5-positive using the POCube system, whereas only 10% of the H5-positive samples were detected as influenza A-positive by an immunochromatography-based rapid diagnostic kit.Conclusions: This novel H5/A kit using POCube is served as a rapid and sensitive screening test for H5N1 HPAI virus infection in humans.
AB - Background: Sporadic emergence of the highly pathogenic avian influenza (HPAI) H5N1 virus infection in humans is a serious concern because of the potential for a pandemic. Conventional or quantitative RT-PCR is the standard laboratory test to detect viral influenza infections. However, this technology requires well-equipped laboratories and highly trained personnel. A rapid, sensitive, and specific alternative screening method is needed.Methods: By a luminescence-linked enzyme immunoassay, we have developed a H5N1 HPAI virus detection kit using anti-H5 hemagglutinin monoclonal antibodies in combination with the detection of a universal NP antigen of the type A influenza virus. The process takes 15 minutes by use of the fully automated luminescence analyzer, POCube.Resutls: We tested this H5/A kit using 19 clinical specimens from 13 patients stored in Vietnam who were infected with clade 1.1 or clade 2.3.4 H5N1 HPAI virus. Approximately 80% of clinical specimens were H5-positive using the POCube system, whereas only 10% of the H5-positive samples were detected as influenza A-positive by an immunochromatography-based rapid diagnostic kit.Conclusions: This novel H5/A kit using POCube is served as a rapid and sensitive screening test for H5N1 HPAI virus infection in humans.
KW - Clinical specimens
KW - H5 hemagglutinin
KW - Highly pathogenic avian influenza virus
KW - Monoclonal antibody
KW - Rapid influenza diagnosis
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U2 - 10.1186/1471-2334-14-362
DO - 10.1186/1471-2334-14-362
M3 - Article
C2 - 24992826
AN - SCOPUS:84907507697
SN - 1471-2334
VL - 14
JO - BMC Infectious Diseases
JF - BMC Infectious Diseases
IS - 1
M1 - 362
ER -
