Development of an antigen-specific CD8 suppressor effector clone in man

Tsutomu Takeuchi, S. F. Schlossman, C. Morimoto

研究成果: Article

9 引用 (Scopus)

抄録

A long-term cultured IL-2-dependent keyhole limpet hemocyanin (KLH)-specific CD8 (T8) suppressor clone (5B9) was generated from a healthy donor hyperimmunized with KLH. The 5B9 clonal population suppressed in vitro anti-KLH antibody response but did not suppress anti-TT antibody response or PWM-driven IgG synthesis. Moreover, 5B9 cells could not suppress anti-TT antibody response even in the presence of free KLH. 5B9 cloned cells suppressed the anti-KLH antibody response of B cells cultured with CD4+4B4+ cells without requiring the presence of CD8+ cells. This KLH-specific CD8 suppressor clone is an effector type rather than an inducer type of suppressor T cell. The cloned cells expressed α- and β-TCR proteins (defined by WT-31 antibody) on their cell surface. More importantly, the CD3:TCR complex was functionally important in the suppression induced by this clone, because after CD3 antigen modulation from its cell surface, the suppressor effector function was abolished.

元の言語English
ページ(範囲)3010-3015
ページ数6
ジャーナルJournal of Immunology
141
発行部数9
出版物ステータスPublished - 1988
外部発表Yes

Fingerprint

CD8 Antigens
Clone Cells
Antibody Formation
Anti-Idiotypic Antibodies
T-Cell Antigen Receptor-CD3 Complex
CD3 Antigens
Interleukin-2
keyhole-limpet hemocyanin
B-Lymphocytes
Immunoglobulin G
Tissue Donors
T-Lymphocytes
Antibodies

ASJC Scopus subject areas

  • Immunology

これを引用

Development of an antigen-specific CD8 suppressor effector clone in man. / Takeuchi, Tsutomu; Schlossman, S. F.; Morimoto, C.

:: Journal of Immunology, 巻 141, 番号 9, 1988, p. 3010-3015.

研究成果: Article

Takeuchi, T, Schlossman, SF & Morimoto, C 1988, 'Development of an antigen-specific CD8 suppressor effector clone in man', Journal of Immunology, 巻. 141, 番号 9, pp. 3010-3015.
Takeuchi, Tsutomu ; Schlossman, S. F. ; Morimoto, C. / Development of an antigen-specific CD8 suppressor effector clone in man. :: Journal of Immunology. 1988 ; 巻 141, 番号 9. pp. 3010-3015.
@article{b0703644265044858818201393da44f7,
title = "Development of an antigen-specific CD8 suppressor effector clone in man",
abstract = "A long-term cultured IL-2-dependent keyhole limpet hemocyanin (KLH)-specific CD8 (T8) suppressor clone (5B9) was generated from a healthy donor hyperimmunized with KLH. The 5B9 clonal population suppressed in vitro anti-KLH antibody response but did not suppress anti-TT antibody response or PWM-driven IgG synthesis. Moreover, 5B9 cells could not suppress anti-TT antibody response even in the presence of free KLH. 5B9 cloned cells suppressed the anti-KLH antibody response of B cells cultured with CD4+4B4+ cells without requiring the presence of CD8+ cells. This KLH-specific CD8 suppressor clone is an effector type rather than an inducer type of suppressor T cell. The cloned cells expressed α- and β-TCR proteins (defined by WT-31 antibody) on their cell surface. More importantly, the CD3:TCR complex was functionally important in the suppression induced by this clone, because after CD3 antigen modulation from its cell surface, the suppressor effector function was abolished.",
author = "Tsutomu Takeuchi and Schlossman, {S. F.} and C. Morimoto",
year = "1988",
language = "English",
volume = "141",
pages = "3010--3015",
journal = "Journal of Immunology",
issn = "0022-1767",
publisher = "American Association of Immunologists",
number = "9",

}

TY - JOUR

T1 - Development of an antigen-specific CD8 suppressor effector clone in man

AU - Takeuchi, Tsutomu

AU - Schlossman, S. F.

AU - Morimoto, C.

PY - 1988

Y1 - 1988

N2 - A long-term cultured IL-2-dependent keyhole limpet hemocyanin (KLH)-specific CD8 (T8) suppressor clone (5B9) was generated from a healthy donor hyperimmunized with KLH. The 5B9 clonal population suppressed in vitro anti-KLH antibody response but did not suppress anti-TT antibody response or PWM-driven IgG synthesis. Moreover, 5B9 cells could not suppress anti-TT antibody response even in the presence of free KLH. 5B9 cloned cells suppressed the anti-KLH antibody response of B cells cultured with CD4+4B4+ cells without requiring the presence of CD8+ cells. This KLH-specific CD8 suppressor clone is an effector type rather than an inducer type of suppressor T cell. The cloned cells expressed α- and β-TCR proteins (defined by WT-31 antibody) on their cell surface. More importantly, the CD3:TCR complex was functionally important in the suppression induced by this clone, because after CD3 antigen modulation from its cell surface, the suppressor effector function was abolished.

AB - A long-term cultured IL-2-dependent keyhole limpet hemocyanin (KLH)-specific CD8 (T8) suppressor clone (5B9) was generated from a healthy donor hyperimmunized with KLH. The 5B9 clonal population suppressed in vitro anti-KLH antibody response but did not suppress anti-TT antibody response or PWM-driven IgG synthesis. Moreover, 5B9 cells could not suppress anti-TT antibody response even in the presence of free KLH. 5B9 cloned cells suppressed the anti-KLH antibody response of B cells cultured with CD4+4B4+ cells without requiring the presence of CD8+ cells. This KLH-specific CD8 suppressor clone is an effector type rather than an inducer type of suppressor T cell. The cloned cells expressed α- and β-TCR proteins (defined by WT-31 antibody) on their cell surface. More importantly, the CD3:TCR complex was functionally important in the suppression induced by this clone, because after CD3 antigen modulation from its cell surface, the suppressor effector function was abolished.

UR - http://www.scopus.com/inward/record.url?scp=0023791550&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0023791550&partnerID=8YFLogxK

M3 - Article

C2 - 2459239

AN - SCOPUS:0023791550

VL - 141

SP - 3010

EP - 3015

JO - Journal of Immunology

JF - Journal of Immunology

SN - 0022-1767

IS - 9

ER -