抄録
A long-term cultured IL-2-dependent keyhole limpet hemocyanin (KLH)-specific CD8 (T8) suppressor clone (5B9) was generated from a healthy donor hyperimmunized with KLH. The 5B9 clonal population suppressed in vitro anti-KLH antibody response but did not suppress anti-TT antibody response or PWM-driven IgG synthesis. Moreover, 5B9 cells could not suppress anti-TT antibody response even in the presence of free KLH. 5B9 cloned cells suppressed the anti-KLH antibody response of B cells cultured with CD4+4B4+ cells without requiring the presence of CD8+ cells. This KLH-specific CD8 suppressor clone is an effector type rather than an inducer type of suppressor T cell. The cloned cells expressed α- and β-TCR proteins (defined by WT-31 antibody) on their cell surface. More importantly, the CD3:TCR complex was functionally important in the suppression induced by this clone, because after CD3 antigen modulation from its cell surface, the suppressor effector function was abolished.
| 元の言語 | English |
|---|---|
| ページ（範囲） | 3010-3015 |
| ページ数 | 6 |
| ジャーナル | Journal of Immunology |
| 巻 | 141 |
| 発行部数 | 9 |
| 出版物ステータス | Published - 1988 |
| 外部発表 | Yes |
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ASJC Scopus subject areas
- Immunology
これを引用
Development of an antigen-specific CD8 suppressor effector clone in man. / Takeuchi, Tsutomu; Schlossman, S. F.; Morimoto, C.
：: Journal of Immunology, 巻 141, 番号 9, 1988, p. 3010-3015.研究成果: Article
}
TY - JOUR
T1 - Development of an antigen-specific CD8 suppressor effector clone in man
AU - Takeuchi, Tsutomu
AU - Schlossman, S. F.
AU - Morimoto, C.
PY - 1988
Y1 - 1988
N2 - A long-term cultured IL-2-dependent keyhole limpet hemocyanin (KLH)-specific CD8 (T8) suppressor clone (5B9) was generated from a healthy donor hyperimmunized with KLH. The 5B9 clonal population suppressed in vitro anti-KLH antibody response but did not suppress anti-TT antibody response or PWM-driven IgG synthesis. Moreover, 5B9 cells could not suppress anti-TT antibody response even in the presence of free KLH. 5B9 cloned cells suppressed the anti-KLH antibody response of B cells cultured with CD4+4B4+ cells without requiring the presence of CD8+ cells. This KLH-specific CD8 suppressor clone is an effector type rather than an inducer type of suppressor T cell. The cloned cells expressed α- and β-TCR proteins (defined by WT-31 antibody) on their cell surface. More importantly, the CD3:TCR complex was functionally important in the suppression induced by this clone, because after CD3 antigen modulation from its cell surface, the suppressor effector function was abolished.
AB - A long-term cultured IL-2-dependent keyhole limpet hemocyanin (KLH)-specific CD8 (T8) suppressor clone (5B9) was generated from a healthy donor hyperimmunized with KLH. The 5B9 clonal population suppressed in vitro anti-KLH antibody response but did not suppress anti-TT antibody response or PWM-driven IgG synthesis. Moreover, 5B9 cells could not suppress anti-TT antibody response even in the presence of free KLH. 5B9 cloned cells suppressed the anti-KLH antibody response of B cells cultured with CD4+4B4+ cells without requiring the presence of CD8+ cells. This KLH-specific CD8 suppressor clone is an effector type rather than an inducer type of suppressor T cell. The cloned cells expressed α- and β-TCR proteins (defined by WT-31 antibody) on their cell surface. More importantly, the CD3:TCR complex was functionally important in the suppression induced by this clone, because after CD3 antigen modulation from its cell surface, the suppressor effector function was abolished.
UR - http://www.scopus.com/inward/record.url?scp=0023791550&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0023791550&partnerID=8YFLogxK
M3 - Article
C2 - 2459239
AN - SCOPUS:0023791550
VL - 141
SP - 3010
EP - 3015
JO - Journal of Immunology
JF - Journal of Immunology
SN - 0022-1767
IS - 9
ER -