Development of an in vivo computer based on Escherichia coli

Hirotaka Nakagawa, Kensaku Sakamoto, Yasubumi Sakakibara

研究成果: Conference contribution

12 被引用数 (Scopus)

抄録

We present a novel framework to develop a programmable and autonomous in vivo computer using E. coli, and implement in vivo finite-state automata based on the framework by employing the protein-synthesis mechanism of E. coli. Our fundamental idea to develop a programmable and autonomous finite-state automata on E. coli is that we first encode an input string into one plasmid, encode state-transition functions into the other plasmid, and introduce those two plasmids into an E. coli cell by electroporation. Second, we execute a protein-synthesis process in E. coli combined with four-base codon techniques to simulate a computation (accepting) process of finite automata, which has been proposed for in vitro translation-based computations in [8]. This approach enables us to develop a programmable in vivo computer by simply replacing a plasmid encoding a state-transition function with others. Further, our in vivo finite automata are autonomous because the protein-synthesis process is autonomously executed in the living E. coli cell. We show some successful experiments to run an in vivo finite-state automaton on E. coli.

本文言語English
ホスト出版物のタイトルDNA Computing - 11th International Workshop on DNA Computing, DNA11, Revised Selected Papers
ページ203-212
ページ数10
DOI
出版ステータスPublished - 2006 7 13
イベント11th International Workshop on DNA Computing, DNA11 - London, ON, Canada
継続期間: 2005 6 62005 6 9

出版物シリーズ

名前Lecture Notes in Computer Science (including subseries Lecture Notes in Artificial Intelligence and Lecture Notes in Bioinformatics)
3892 LNCS
ISSN(印刷版)0302-9743
ISSN(電子版)1611-3349

Other

Other11th International Workshop on DNA Computing, DNA11
国/地域Canada
CityLondon, ON
Period05/6/605/6/9

ASJC Scopus subject areas

  • 理論的コンピュータサイエンス
  • コンピュータ サイエンス(全般)

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