Development of cell-impermeable coelenterazine derivatives

Eric Lindberg, Shin Mizukami, Keiji Ibata, Takashi Fukano, Atsushi Miyawaki, Kazuya Kikuchi

研究成果: Article査読

15 被引用数 (Scopus)

抄録

We describe the development of the first cell-membrane impermeable coelenterazine derivative (CoelPhos). CoelPhos was constructed by the alkylation of coelenterazine with a linker containing a terminal anionic phosphonate moiety. The bioluminescence activity of CoelPhos with Gaussia luciferase (GLuc) showed a significantly higher activity in comparison with Renilla luciferase. In imaging studies with living cells, outer membrane bound GLuc was clearly imaged with CoelPhos. On the other hand no signal could be detected with intracellularly localized GLuc, demonstrating the impermeability of this novel coelenterate substrate derivative. CoelPhos has potential utility as a new bioluminogenic tool for the monitoring of dynamic fusion events at the cell–surface interface.

本文言語English
ページ(範囲)4395-4400
ページ数6
ジャーナルChemical Science
4
12
DOI
出版ステータスPublished - 2013 10月 28

ASJC Scopus subject areas

  • 化学 (全般)

フィンガープリント

「Development of cell-impermeable coelenterazine derivatives」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル