Development of Highly Selective Fluorescent Probe Enabling Flow-Cytometric Isolation of ALDH3A1-Positive Viable Cells

Atsushi Yagishita; Tasuku Ueno; Hiroyasu Esumi; Hideyuki Saya; Kazuhiro Kaneko; Katsuya Tsuchihara; Yasuteru Urano

doi:10.1021/acs.bioconjchem.6b00618

Development of Highly Selective Fluorescent Probe Enabling Flow-Cytometric Isolation of ALDH3A1-Positive Viable Cells

Atsushi Yagishita, Tasuku Ueno, Hiroyasu Esumi, Hideyuki Saya, Kazuhiro Kaneko, Katsuya Tsuchihara, Yasuteru Urano

先端医科学研究所(遺伝子)

研究成果: Article › 査読

7 被引用数 (Scopus)

抄録

Aldehyde dehydrogenase (ALDH) is overexpressed in some subpopulations of stem cells and cancer cells. We have designed and synthesized the first selective fluorescent probe for class 3 ALDH (ALDH3A1). This probe enabled the visualization of ALDH3A1-positive cells by fluorescence microscopy as well as flow-cytometric isolation of ALDH3A1-positive viable cells from a human Caucasian esophageal squamous cell line (OE21) that heterogeneously expresses ALDH3A1.

本文言語	English
ページ（範囲）	302-306
ページ数	5
ジャーナル	Bioconjugate Chemistry
巻	28
号	2
DOI	https://doi.org/10.1021/acs.bioconjchem.6b00618
出版ステータス	Published - 2017 2月 15

ASJC Scopus subject areas

バイオテクノロジー
バイオエンジニアリング
生体医工学
薬理学
薬科学
有機化学

UN SDG

この成果は、次の持続可能な開発目標に貢献しています

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10.1021/acs.bioconjchem.6b00618

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title = "Development of Highly Selective Fluorescent Probe Enabling Flow-Cytometric Isolation of ALDH3A1-Positive Viable Cells",

abstract = "Aldehyde dehydrogenase (ALDH) is overexpressed in some subpopulations of stem cells and cancer cells. We have designed and synthesized the first selective fluorescent probe for class 3 ALDH (ALDH3A1). This probe enabled the visualization of ALDH3A1-positive cells by fluorescence microscopy as well as flow-cytometric isolation of ALDH3A1-positive viable cells from a human Caucasian esophageal squamous cell line (OE21) that heterogeneously expresses ALDH3A1.",

author = "Atsushi Yagishita and Tasuku Ueno and Hiroyasu Esumi and Hideyuki Saya and Kazuhiro Kaneko and Katsuya Tsuchihara and Yasuteru Urano",

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year = "2017",

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day = "15",

doi = "10.1021/acs.bioconjchem.6b00618",

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AU - Yagishita, Atsushi

AU - Ueno, Tasuku

AU - Esumi, Hiroyasu

AU - Saya, Hideyuki

AU - Kaneko, Kazuhiro

AU - Tsuchihara, Katsuya

AU - Urano, Yasuteru

PY - 2017/2/15

Y1 - 2017/2/15

N2 - Aldehyde dehydrogenase (ALDH) is overexpressed in some subpopulations of stem cells and cancer cells. We have designed and synthesized the first selective fluorescent probe for class 3 ALDH (ALDH3A1). This probe enabled the visualization of ALDH3A1-positive cells by fluorescence microscopy as well as flow-cytometric isolation of ALDH3A1-positive viable cells from a human Caucasian esophageal squamous cell line (OE21) that heterogeneously expresses ALDH3A1.

AB - Aldehyde dehydrogenase (ALDH) is overexpressed in some subpopulations of stem cells and cancer cells. We have designed and synthesized the first selective fluorescent probe for class 3 ALDH (ALDH3A1). This probe enabled the visualization of ALDH3A1-positive cells by fluorescence microscopy as well as flow-cytometric isolation of ALDH3A1-positive viable cells from a human Caucasian esophageal squamous cell line (OE21) that heterogeneously expresses ALDH3A1.

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AN - SCOPUS:85013087521

SN - 1043-1802

VL - 28

SP - 302

EP - 306

JO - Bioconjugate Chemistry

JF - Bioconjugate Chemistry

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Development of Highly Selective Fluorescent Probe Enabling Flow-Cytometric Isolation of ALDH3A1-Positive Viable Cells

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ASJC Scopus subject areas

UN SDG

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