Development of in vivo drug sensing system with needle-type diamond microelectrode

Genki Ogata, Kai Asai, Yamato Sano, Seishiro Sawamura, Madoka Takai, Hiroyuki Kusuhara, Yasuaki Einaga, Hiroshi Hibino

研究成果: Article査読

抄録

Continuous and real-time measurement of local concentrations of systemically administered drugs in vivo must be crucial for pharmacological studies. Nevertheless, conventional methods require considerable samples quantity and have poor sampling rates. Additionally, they cannot determine how drug kinetics correlates with target function over time. Here, we describe a system with two different sensors. One is a needle-type microsensor composed of boron-doped diamond with a tip of ~40 μm in diameter, and the other is a glass microelectrode. We first tested bumetanide. This diuretic can induce deafness. In the guinea-pig cochlea injected intravenously with bumetanide, the changes of the drug concentration and the extracellular potential underlying hearing were simultaneously measured in real time. We further examined an antiepileptic drug lamotrigine in the rat brain, and tracked its kinetics and at the same time the local field potentials representing neuronal activity. The action of the anticancer reagent doxorubicin was also monitored in the cochlea. This microsensing system may be applied to analyze pharmacokinetics and pharmacodynamics of various drugs at local sites in vivo, and contribute to promoting the pharmacological researches.

本文言語English
ページ(範囲)273-277
ページ数5
ジャーナルFolia Pharmacologica Japonica
153
6
DOI
出版ステータスPublished - 2019

ASJC Scopus subject areas

  • 薬理学

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