Development of microsatellite markers for the Japanese giant salamander (Andrias japonicus) through next-generation sequencing, and cross-amplification in its congener

Natsuhiko Yoshikawa, Masafumi Matsui, Azusa Hayano, Miho Inoue-Murayama

研究成果: Article査読

6 被引用数 (Scopus)

抄録

A giant salamander Andrias japonicus, endemic to western Japan is ranked as near threatened. However, genetic markers necessary for estimating genetic diversity and structures for conservation measures have been limited. Therefore, we developed seventeen novel microsatellite markers from A. japonicus, and tried cross-amplification in its congener, Andrias davidianus. The number of alleles and expected heterozygosity at each locus were 1-5 (mean = 2. 53) and 0-0. 66, respectively, in A. japonicus. Cross-amplification in A. davidianus succeeded in 15 of 17 loci. The markers described here will be useful for investigating the genetic diversity and genetic structure, and planning conservation management of A. japonicus and A. davidianus.

本文言語English
ページ(範囲)971-974
ページ数4
ジャーナルConservation Genetics Resources
4
4
DOI
出版ステータスPublished - 2012 12月
外部発表はい

ASJC Scopus subject areas

  • 生態、進化、行動および分類学
  • 遺伝学

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