Development of Near-Infrared Fluorescent Mg2+ Probe and Application to Multicolor Imaging of Intracellular Signals

Yutaka Shindo, Yuma Ikeda, Yuki Hiruta, Daniel Citterio, Kotaro Oka

研究成果: Chapter

抄録

Recent extensive studies revealed that the intracellular concentration of magnesium ions (Mg2+) is one of the important factors to regulate cellular functions. To evaluate the impact of Mg2+ concentration changes on intracellular signals or events, simultaneous imaging of Mg2+ with those phenomena is a powerful technique. The present protocol describes the synthesis and evaluation of near-infrared (NIR) fluorescent Mg2+-selective probes, named KMG-500 series, and the application to simultaneous imaging of the corresponding intracellular signal transductions and molecular events. The present protocol for multicolor imaging using fluorescent probes in the NIR and visible ranges is highly useful to reveal how multiple molecular events are correlated each other in each single cell.

本文言語English
ホスト出版物のタイトルMethods in Molecular Biology
出版社Humana Press Inc.
ページ217-235
ページ数19
DOI
出版ステータスPublished - 2021

出版物シリーズ

名前Methods in Molecular Biology
2274
ISSN(印刷版)1064-3745
ISSN(電子版)1940-6029

ASJC Scopus subject areas

  • 分子生物学
  • 遺伝学

フィンガープリント

「Development of Near-Infrared Fluorescent Mg<sup>2+</sup> Probe and Application to Multicolor Imaging of Intracellular Signals」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル