Development of neuraminidase detection using gold nanoparticles boron-doped diamond electrodes

Wulan T. Wahyuni, Tribidasari A. Ivandini, Endang Saepudin, Yasuaki Einaga

研究成果: Article査読

10 被引用数 (Scopus)

抄録

Gold nanoparticles-modified boron-doped diamond (AuNPs-BDD) electrodes, which were prepared with a self-assembly deposition of AuNPs at amine-terminated boron-doped diamond, were examined for voltammetric detection of neuraminidase (NA). The detection method was performed based on the difference of electrochemical responses of zanamivir at gold surface before and after the reaction with NA in phosphate buffer solution (PBS, pH 5.5). A linear calibration curve for zanamivir in 0.1 M PBS in the absence of NA was achieved in the concentration range of 1 × 10-6 to 1 × 10-5 M (R2 = 0.99) with an estimated limit of detection (LOD) of 2.29 × 10-6 M. Furthermore, using its reaction with 1.00 × 10-5 M zanamivir, a linear calibration curve of NA can be obtained in the concentration range of 0-12 mU (R2 = 0.99) with an estimated LOD of 0.12 mU. High reproducibility was shown with a relative standard deviation (RSD) of 1.14% (n = 30). These performances could be maintained when the detection was performed in mucin matrix. Comparison performed using gold-modified BDD (Au-BDD) electrodes suggested that the good performance of the detection method is due to the stability of the gold particles position at the BDD surface.

本文言語English
ページ(範囲)68-75
ページ数8
ジャーナルAnalytical Biochemistry
497
DOI
出版ステータスPublished - 2016 3 15

ASJC Scopus subject areas

  • 生物理学
  • 生化学
  • 分子生物学
  • 細胞生物学

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