Development of new method to enrich human iPSC-derived renal progenitors using cell surface markers

Azusa Hoshina, Tatsuya Kawamoto, Shin Ichi Sueta, Shin Ichi Mae, Toshikazu Araoka, Hiromi Tanaka, Yasunori Sato, Yukiko Yamagishi, Kenji Osafune

研究成果: Article査読

20 被引用数 (Scopus)

抄録

Cell therapy using renal progenitors differentiated from human embryonic stem cells (hESCs) or induced pluripotent stem cells (hiPSCs) has the potential to significantly reduce the number of patients receiving dialysis therapy. However, the differentiation cultures may contain undifferentiated or undesired cell types that cause unwanted side effects, such as neoplastic formation, when transplanted into a body. Moreover, the hESCs/iPSCs are often genetically modified in order to isolate the derived renal progenitors, hampering clinical applications. To establish an isolation method for renal progenitors induced from hESCs/iPSCs without genetic modifications, we screened antibodies against cell surface markers. We identified the combination of four markers, CD9-CD140a+CD140b+CD271+, which could enrich OSR1+SIX2+ renal progenitors. Furthermore, these isolated cells ameliorated renal injury in an acute kidney injury (AKI) mouse model when used for cell therapy. These cells could contribute to the development of hiPSC-based cell therapy and disease modeling against kidney diseases.

本文言語English
論文番号6375
ジャーナルScientific reports
8
1
DOI
出版ステータスPublished - 2018 12月 1
外部発表はい

ASJC Scopus subject areas

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