TY - JOUR
T1 - Development of novel monoclonal antibody 4G8 against swine leukocyte antigen class I α chain
AU - Tang, Wei Ran
AU - Kiyokawa, Nobutaka
AU - Eguchi, Tomoko
AU - Matsui, Jun
AU - Takenouchi, Hisami
AU - Honma, Daisuk
AU - Yasue, Hiroshi
AU - Enosawa, Shin
AU - Mimori, Kenichi
AU - Itagaki, Mitsuko
AU - Taguchi, Tomoko
AU - Katagiri, Yohko U.
AU - Okita, Hajime
AU - Amemiya, Hiroshi
AU - Fujimoto, Junichiro
PY - 2004/6
Y1 - 2004/6
N2 - A mouse monoclonal antibody (MAb) was generated against swine leukocyte antigen (SLA) class I α chain. A newly developed series of MAb clones that react with pan leukocytes were selected and tested by immunohistochemistry using SLA class I α chain expressing Cos-7 cells. Among them, MAb 4G8 was characterized by the following features: (1) 4G8 reacted with Cos-7 cells transfected with SLA class I α chain from the d haplotype, (2) 4G8 recognized epitopes that were different from those of commercially available anti-SLA class I MAbs 74-11-10 and PT85A, and (3) 4G8 could be used to immunostain frozen sections of thymus, spleen, lymph node, kidney, and liver tissues with good results.
AB - A mouse monoclonal antibody (MAb) was generated against swine leukocyte antigen (SLA) class I α chain. A newly developed series of MAb clones that react with pan leukocytes were selected and tested by immunohistochemistry using SLA class I α chain expressing Cos-7 cells. Among them, MAb 4G8 was characterized by the following features: (1) 4G8 reacted with Cos-7 cells transfected with SLA class I α chain from the d haplotype, (2) 4G8 recognized epitopes that were different from those of commercially available anti-SLA class I MAbs 74-11-10 and PT85A, and (3) 4G8 could be used to immunostain frozen sections of thymus, spleen, lymph node, kidney, and liver tissues with good results.
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U2 - 10.1089/1536859041224253
DO - 10.1089/1536859041224253
M3 - Article
C2 - 15312310
AN - SCOPUS:3042711395
VL - 23
SP - 187
EP - 191
JO - Monoclonal Antibodies in Immunodiagnosis and Immunotherapy
JF - Monoclonal Antibodies in Immunodiagnosis and Immunotherapy
SN - 2167-9436
IS - 3
ER -