Development of novel monoclonal antibody 4G8 against swine leukocyte antigen class I α chain

Wei Ran Tang; Nobutaka Kiyokawa; Tomoko Eguchi; Jun Matsui; Hisami Takenouchi; Daisuk Honma; Hiroshi Yasue; Shin Enosawa; Kenichi Mimori; Mitsuko Itagaki; Tomoko Taguchi; Yohko U. Katagiri; Hajime Okita; Hiroshi Amemiya; Junichiro Fujimoto

doi:10.1089/1536859041224253

Development of novel monoclonal antibody 4G8 against swine leukocyte antigen class I α chain

Wei Ran Tang, Nobutaka Kiyokawa, Tomoko Eguchi, Jun Matsui, Hisami Takenouchi, Daisuk Honma, Hiroshi Yasue, Shin Enosawa, Kenichi Mimori, Mitsuko Itagaki, Tomoko Taguchi, Yohko U. Katagiri, Hajime Okita, Hiroshi Amemiya, Junichiro Fujimoto

研究成果: Article › 査読

4 被引用数 (Scopus)

抄録

A mouse monoclonal antibody (MAb) was generated against swine leukocyte antigen (SLA) class I α chain. A newly developed series of MAb clones that react with pan leukocytes were selected and tested by immunohistochemistry using SLA class I α chain expressing Cos-7 cells. Among them, MAb 4G8 was characterized by the following features: (1) 4G8 reacted with Cos-7 cells transfected with SLA class I α chain from the d haplotype, (2) 4G8 recognized epitopes that were different from those of commercially available anti-SLA class I MAbs 74-11-10 and PT85A, and (3) 4G8 could be used to immunostain frozen sections of thymus, spleen, lymph node, kidney, and liver tissues with good results.

本文言語	English
ページ（範囲）	187-191
ページ数	5
ジャーナル	Hybridoma and Hybridomics
巻	23
号	3
DOI	https://doi.org/10.1089/1536859041224253
出版ステータス	Published - 2004 6
外部発表	はい

ASJC Scopus subject areas

免疫学
遺伝学

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10.1089/1536859041224253

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引用スタイル

Tang, W. R., Kiyokawa, N., Eguchi, T., Matsui, J., Takenouchi, H., Honma, D., Yasue, H., Enosawa, S., Mimori, K., Itagaki, M., Taguchi, T., Katagiri, Y. U., Okita, H., Amemiya, H., & Fujimoto, J. (2004). Development of novel monoclonal antibody 4G8 against swine leukocyte antigen class I α chain. Hybridoma and Hybridomics, 23(3), 187-191. https://doi.org/10.1089/1536859041224253

Development of novel monoclonal antibody 4G8 against swine leukocyte antigen class I α chain. / Tang, Wei Ran; Kiyokawa, Nobutaka; Eguchi, Tomoko; Matsui, Jun; Takenouchi, Hisami; Honma, Daisuk; Yasue, Hiroshi; Enosawa, Shin; Mimori, Kenichi; Itagaki, Mitsuko; Taguchi, Tomoko; Katagiri, Yohko U.; Okita, Hajime; Amemiya, Hiroshi; Fujimoto, Junichiro.

In: Hybridoma and Hybridomics, Vol. 23, No. 3, 06.2004, p. 187-191.

研究成果: Article › 査読

Tang, WR, Kiyokawa, N, Eguchi, T, Matsui, J, Takenouchi, H, Honma, D, Yasue, H, Enosawa, S, Mimori, K, Itagaki, M, Taguchi, T, Katagiri, YU, Okita, H, Amemiya, H & Fujimoto, J 2004, 'Development of novel monoclonal antibody 4G8 against swine leukocyte antigen class I α chain', Hybridoma and Hybridomics, vol. 23, no. 3, pp. 187-191. https://doi.org/10.1089/1536859041224253

Tang, Wei Ran ; Kiyokawa, Nobutaka ; Eguchi, Tomoko ; Matsui, Jun ; Takenouchi, Hisami ; Honma, Daisuk ; Yasue, Hiroshi ; Enosawa, Shin ; Mimori, Kenichi ; Itagaki, Mitsuko ; Taguchi, Tomoko ; Katagiri, Yohko U. ; Okita, Hajime ; Amemiya, Hiroshi ; Fujimoto, Junichiro. / Development of novel monoclonal antibody 4G8 against swine leukocyte antigen class I α chain. In: Hybridoma and Hybridomics. 2004 ; Vol. 23, No. 3. pp. 187-191.

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abstract = "A mouse monoclonal antibody (MAb) was generated against swine leukocyte antigen (SLA) class I α chain. A newly developed series of MAb clones that react with pan leukocytes were selected and tested by immunohistochemistry using SLA class I α chain expressing Cos-7 cells. Among them, MAb 4G8 was characterized by the following features: (1) 4G8 reacted with Cos-7 cells transfected with SLA class I α chain from the d haplotype, (2) 4G8 recognized epitopes that were different from those of commercially available anti-SLA class I MAbs 74-11-10 and PT85A, and (3) 4G8 could be used to immunostain frozen sections of thymus, spleen, lymph node, kidney, and liver tissues with good results.",

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AU - Takenouchi, Hisami

AU - Honma, Daisuk

AU - Yasue, Hiroshi

AU - Enosawa, Shin

AU - Mimori, Kenichi

AU - Itagaki, Mitsuko

AU - Taguchi, Tomoko

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AU - Okita, Hajime

AU - Amemiya, Hiroshi

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Development of novel monoclonal antibody 4G8 against swine leukocyte antigen class I α chain

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