Development of novel monoclonal antibody 4G8 against swine leukocyte antigen class I α chain

Wei Ran Tang; Nobutaka Kiyokawa; Tomoko Eguchi; Jun Matsui; Hisami Takenouchi; Daisuk Honma; Hiroshi Yasue; Shin Enosawa; Kenichi Mimori; Mitsuko Itagaki; Tomoko Taguchi; Yohko U. Katagiri; Hajime Okita; Hiroshi Amemiya; Junichiro Fujimoto

doi:10.1089/1536859041224253

Development of novel monoclonal antibody 4G8 against swine leukocyte antigen class I α chain

Wei Ran Tang, Nobutaka Kiyokawa, Tomoko Eguchi, Jun Matsui, Hisami Takenouchi, Daisuk Honma, Hiroshi Yasue, Shin Enosawa, Kenichi Mimori, Mitsuko Itagaki, Tomoko Taguchi, Yohko U. Katagiri, Hajime Okita, Hiroshi Amemiya, Junichiro Fujimoto

研究成果: Article › 査読

4 被引用数 (Scopus)

抄録

A mouse monoclonal antibody (MAb) was generated against swine leukocyte antigen (SLA) class I α chain. A newly developed series of MAb clones that react with pan leukocytes were selected and tested by immunohistochemistry using SLA class I α chain expressing Cos-7 cells. Among them, MAb 4G8 was characterized by the following features: (1) 4G8 reacted with Cos-7 cells transfected with SLA class I α chain from the d haplotype, (2) 4G8 recognized epitopes that were different from those of commercially available anti-SLA class I MAbs 74-11-10 and PT85A, and (3) 4G8 could be used to immunostain frozen sections of thymus, spleen, lymph node, kidney, and liver tissues with good results.

本文言語	English
ページ（範囲）	187-191
ページ数	5
ジャーナル	Hybridoma and Hybridomics
巻	23
号	3
DOI	https://doi.org/10.1089/1536859041224253
出版ステータス	Published - 2004 6月
外部発表	はい

ASJC Scopus subject areas

免疫学
遺伝学

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10.1089/1536859041224253

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Tang, W. R., Kiyokawa, N., Eguchi, T., Matsui, J., Takenouchi, H., Honma, D., Yasue, H., Enosawa, S., Mimori, K., Itagaki, M., Taguchi, T., Katagiri, Y. U., Okita, H., Amemiya, H., & Fujimoto, J. (2004). Development of novel monoclonal antibody 4G8 against swine leukocyte antigen class I α chain. Hybridoma and Hybridomics, 23(3), 187-191. https://doi.org/10.1089/1536859041224253

Tang, WR, Kiyokawa, N, Eguchi, T, Matsui, J, Takenouchi, H, Honma, D, Yasue, H, Enosawa, S, Mimori, K, Itagaki, M, Taguchi, T, Katagiri, YU, Okita, H, Amemiya, H & Fujimoto, J 2004, 'Development of novel monoclonal antibody 4G8 against swine leukocyte antigen class I α chain', Hybridoma and Hybridomics, vol. 23, no. 3, pp. 187-191. https://doi.org/10.1089/1536859041224253

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abstract = "A mouse monoclonal antibody (MAb) was generated against swine leukocyte antigen (SLA) class I α chain. A newly developed series of MAb clones that react with pan leukocytes were selected and tested by immunohistochemistry using SLA class I α chain expressing Cos-7 cells. Among them, MAb 4G8 was characterized by the following features: (1) 4G8 reacted with Cos-7 cells transfected with SLA class I α chain from the d haplotype, (2) 4G8 recognized epitopes that were different from those of commercially available anti-SLA class I MAbs 74-11-10 and PT85A, and (3) 4G8 could be used to immunostain frozen sections of thymus, spleen, lymph node, kidney, and liver tissues with good results.",

author = "Tang, {Wei Ran} and Nobutaka Kiyokawa and Tomoko Eguchi and Jun Matsui and Hisami Takenouchi and Daisuk Honma and Hiroshi Yasue and Shin Enosawa and Kenichi Mimori and Mitsuko Itagaki and Tomoko Taguchi and Katagiri, {Yohko U.} and Hajime Okita and Hiroshi Amemiya and Junichiro Fujimoto",

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doi = "10.1089/1536859041224253",

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AU - Tang, Wei Ran

AU - Kiyokawa, Nobutaka

AU - Eguchi, Tomoko

AU - Matsui, Jun

AU - Takenouchi, Hisami

AU - Honma, Daisuk

AU - Yasue, Hiroshi

AU - Enosawa, Shin

AU - Mimori, Kenichi

AU - Itagaki, Mitsuko

AU - Taguchi, Tomoko

AU - Katagiri, Yohko U.

AU - Okita, Hajime

AU - Amemiya, Hiroshi

AU - Fujimoto, Junichiro

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AB - A mouse monoclonal antibody (MAb) was generated against swine leukocyte antigen (SLA) class I α chain. A newly developed series of MAb clones that react with pan leukocytes were selected and tested by immunohistochemistry using SLA class I α chain expressing Cos-7 cells. Among them, MAb 4G8 was characterized by the following features: (1) 4G8 reacted with Cos-7 cells transfected with SLA class I α chain from the d haplotype, (2) 4G8 recognized epitopes that were different from those of commercially available anti-SLA class I MAbs 74-11-10 and PT85A, and (3) 4G8 could be used to immunostain frozen sections of thymus, spleen, lymph node, kidney, and liver tissues with good results.

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Development of novel monoclonal antibody 4G8 against swine leukocyte antigen class I α chain

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