Development of novel monoclonal antibody 4G8 against swine leukocyte antigen class I α chain

Wei Ran Tang, Nobutaka Kiyokawa, Tomoko Eguchi, Jun Matsui, Hisami Takenouchi, Daisuk Honma, Hiroshi Yasue, Shin Enosawa, Kenichi Mimori, Mitsuko Itagaki, Tomoko Taguchi, Yohko U. Katagiri, Hajime Okita, Hiroshi Amemiya, Junichiro Fujimoto

研究成果: Article査読

4 被引用数 (Scopus)

抄録

A mouse monoclonal antibody (MAb) was generated against swine leukocyte antigen (SLA) class I α chain. A newly developed series of MAb clones that react with pan leukocytes were selected and tested by immunohistochemistry using SLA class I α chain expressing Cos-7 cells. Among them, MAb 4G8 was characterized by the following features: (1) 4G8 reacted with Cos-7 cells transfected with SLA class I α chain from the d haplotype, (2) 4G8 recognized epitopes that were different from those of commercially available anti-SLA class I MAbs 74-11-10 and PT85A, and (3) 4G8 could be used to immunostain frozen sections of thymus, spleen, lymph node, kidney, and liver tissues with good results.

本文言語English
ページ(範囲)187-191
ページ数5
ジャーナルHybridoma and Hybridomics
23
3
DOI
出版ステータスPublished - 2004 6 1
外部発表はい

ASJC Scopus subject areas

  • Immunology
  • Genetics

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