Development of targeted therapy with paclitaxel incorporated into EGF-conjugated nanoparticles

Toshiyuki Shimada, Masakazu Ueda, Hiromitsu Jinno, Naokazu Chiba, Masahiro Wada, Junji Watanabe, Kazuhiko Ishihara, Yuko Kitagawa

研究成果: Article査読

34 被引用数 (Scopus)

抄録

Background: 2-Methacryloyloxyethyl phosphorylcholine (MPC) polymer is a suitable vehicle for paclitaxel (PTX) delivery. A new targeted therapy has been developed by conjugating epidermal growth factor (EGF) to MPC polymer and its growth inhibitory and antitumor effects on cancer cells overexpressing EGF receptors (EGFR) has been investigated. Materials and Methods: EGF was conjugated to poly [MPC-co-n-butyl methacrylate-co-p-nitrophenylo- xycarbonyl poly (ethylene glycol) methacrylate] (PMBN) and mixed with PTX. The cytotoxicity of the resulting PTX incorporated into EGF-conjugated PMBN (EGF-PMBN-PTX) on EGFR-overexpressing and EGFR-deficient cell lines was compared with PTX incorporated into PMBN alone (PMBN-PTX) and PTX alone. Suspensions of the cells were injected into nude mice subcutaneously. EGF-PMBN-PTX, PMBN- PTX, PTX or NaCl solution was injected intraperitoneally. Results: The cytotoxicity and antitumor effect of EGF-PMBN- PTX were significantly greater than those of PMBN-PTX for EGFR-overexpressing cells but not for an EGFR-deficient line. Conclusion: These results suggest that EGF-PMBN-PTX may represent a more potent targeted therapy for tumors overexpressing EGFR.

本文言語English
ページ(範囲)1009-1014
ページ数6
ジャーナルAnticancer research
29
4
出版ステータスPublished - 2009 4

ASJC Scopus subject areas

  • 腫瘍学
  • 癌研究

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