Development of UV-excitable red and near-infrared fluorescent labels and their application for simultaneous multicolor bioimaging by single-wavelength excitation

Tetsuya Mizuno, Keitaro Umezawa, Yutaka Shindo, Daniel Citterio, Kotaro Oka, Koji Suzuki

研究成果: Article査読

7 被引用数 (Scopus)

抄録

We report a new type of UV-excitable red/NIR-emissive fluorescent dyads (PKF series). Conjugation of a pyrene and a novel bright red/near-infrared (NIR) fluorophore resulted in large quasi-Stokes shift while retaining intense fluorescence emission and sharp spectral bands. Labeling of PKF dyads to biomolecules was performed by means of introduction of a succinimidyl ester. Simultaneous Ca2+/albumin dual-color intracellular imaging by PKF in combination with fura-2 (UV-excitable/VIS-emissive Ca2+ indicator) reveals its usefulness as a new bioimaging tool.

本文言語English
ページ(範囲)1007-1018
ページ数12
ジャーナルJournal of Fluorescence
23
5
DOI
出版ステータスPublished - 2013 9

ASJC Scopus subject areas

  • 生化学
  • 臨床心理学
  • 社会科学(その他)
  • 社会学および政治科学
  • 分光学
  • 臨床生化学
  • 法学

フィンガープリント

「Development of UV-excitable red and near-infrared fluorescent labels and their application for simultaneous multicolor bioimaging by single-wavelength excitation」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル