We report a new type of UV-excitable red/NIR-emissive fluorescent dyads (PKF series). Conjugation of a pyrene and a novel bright red/near-infrared (NIR) fluorophore resulted in large quasi-Stokes shift while retaining intense fluorescence emission and sharp spectral bands. Labeling of PKF dyads to biomolecules was performed by means of introduction of a succinimidyl ester. Simultaneous Ca2+/albumin dual-color intracellular imaging by PKF in combination with fura-2 (UV-excitable/VIS-emissive Ca2+ indicator) reveals its usefulness as a new bioimaging tool.
ASJC Scopus subject areas
- Clinical Psychology
- Social Sciences (miscellaneous)
- Sociology and Political Science
- Clinical Biochemistry