@article{d9c655ae91504ea6aceee1e3d7e7fc2b,
title = "Development of UV-excitable red and near-infrared fluorescent labels and their application for simultaneous multicolor bioimaging by single-wavelength excitation",
abstract = "We report a new type of UV-excitable red/NIR-emissive fluorescent dyads (PKF series). Conjugation of a pyrene and a novel bright red/near-infrared (NIR) fluorophore resulted in large quasi-Stokes shift while retaining intense fluorescence emission and sharp spectral bands. Labeling of PKF dyads to biomolecules was performed by means of introduction of a succinimidyl ester. Simultaneous Ca2+/albumin dual-color intracellular imaging by PKF in combination with fura-2 (UV-excitable/VIS-emissive Ca2+ indicator) reveals its usefulness as a new bioimaging tool.",
keywords = "BODIPY, Bioimaging, Fluorescent dyads, Near-infrared fluorophore",
author = "Tetsuya Mizuno and Keitaro Umezawa and Yutaka Shindo and Daniel Citterio and Kotaro Oka and Koji Suzuki",
note = "Funding Information: Acknowledgement We acknowledge T. Shichi (Nissan Arc. Ltd.) for his help with ESI-MS data acquisition, and Y. Urano (The University of Tokyo) for his support for fluorescence lifetime measurements. This work was supported by a JSPS stipend to K.U. and a Grant-in-Aid for Scientific Research (A) from the Ministry of Education, Culture, Sports, Science and Technology, Japan (No. 20245019).",
year = "2013",
month = sep,
doi = "10.1007/s10895-013-1227-5",
language = "English",
volume = "23",
pages = "1007--1018",
journal = "Journal of Fluorescence",
issn = "1053-0509",
publisher = "Springer New York",
number = "5",
}