Diagnosis and prognostication of ductal adenocarcinomas of the pancreas based on genome-wide DNA methylation profiling by bacterial artificial chromosome array-based methylated CpG island amplification

Yae Kanai, Masahiro Gotoh, Eri Arai, Saori Wakai-Ushijima, Nobuyoshi Hiraoka, Tomoo Kosuge, Fumie Hosoda, Tatsuhiro Shibata, Tadashi Kondo, Sana Yokoi, Issei Imoto, Johji Inazawa

研究成果: Article査読

5 被引用数 (Scopus)

抄録

To establish diagnostic criteria for ductal adenocarcinomas of the pancreas (PCs), bacterial artificial chromosome (BAC) array-based methylated CpG island amplification was performed using 139 tissue samples. Twelve BAC clones, for which DNA methylation status was able to discriminate cancerous tissue (T) from noncancerous pancreatic tissue in the learning cohort with a specificity of 100, were identified. Using criteria that combined the 12 BAC clones, T-samples were diagnosed as cancers with 100 sensitivity and specificity in both the learning and validation cohorts. DNA methylation status on 11 of the BAC clones, which was able to discriminate patients showing early relapse from those with no relapse in the learning cohort with 100 specificity, was correlated with the recurrence-free and overall survival rates in the validation cohort and was an independent prognostic factor by multivariate analysis. Genome-wide DNA methylation profiling may provide optimal diagnostic markers and prognostic indicators for patients with PCs.

本文言語English
論文番号780836
ジャーナルJournal of Biomedicine and Biotechnology
2011
DOI
出版ステータスPublished - 2011
外部発表はい

ASJC Scopus subject areas

  • バイオテクノロジー
  • 分子医療
  • 分子生物学
  • 遺伝学
  • 健康、毒物学および変異誘発

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