Diced electrophoresis gel assay for screening enzymes with specified activities

Toru Komatsu, Kenjiro Hanaoka, Alexander Adibekian, Kentaro Yoshioka, Takuya Terai, Tasuku Ueno, Mitsuyasu Kawaguchi, Benjamin F. Cravatt, Tetsuo Nagano

研究成果: Article査読

22 被引用数 (Scopus)

抄録

We have established the diced electrophoresis gel (DEG) assay as a proteome-wide screening tool to identify enzymes with activities of interest using turnover-based fluorescent substrates. The method utilizes the combination of native polyacrylamide gel electrophoresis (PAGE) with a multiwell-plate- based fluorometric assay to find protein spots with the specified activity. By developing fluorescent substrates that mimic the structure of neutrophil chemoattractants, we could identify enzymes involved in metabolic inactivation of the chemoattractants.

本文言語English
ページ(範囲)6002-6005
ページ数4
ジャーナルJournal of the American Chemical Society
135
16
DOI
出版ステータスPublished - 2013 4月 24
外部発表はい

ASJC Scopus subject areas

  • 触媒
  • 化学 (全般)
  • 生化学
  • コロイド化学および表面化学

フィンガープリント

「Diced electrophoresis gel assay for screening enzymes with specified activities」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル