Dictyostelium differentiation-inducing factor-1 promotes glucose uptake, at least in part, via an ampk-dependent pathway in mouse 3t3-l1 cells

Yuzuru Kubohara, Yoshimi Homma, Hiroshi Shibata, Yoshiteru Oshima, Haruhisa Kikuchi

研究成果: Article査読

抄録

Differentiation-inducing factor-1 (DIF-1) is a chlorinated alkylphenone (a polyketide) found in the cellular slime mold Dictyostelium discoideum. DIF-1 and its derivative, DIF-1(3M) promote glucose consumption in vitro in mammalian cells and in vivo in diabetic rats; they are expected to be the leading antiobesity and antidiabetes compounds. In this study, we investigated the mechanisms underlying the actions of DIF-1 and DIF-1(3M). In isolated mouse liver mitochondria, these compounds at 2–20 μM promoted oxygen consumption in a dose-dependent manner, suggesting that they act as mitochondrial uncouplers, whereas CP-DIF-1 (another derivative of DIF-1) at 10–20 μM had no effect. In confluent mouse 3T3-L1 fibroblasts, DIF-1 and DIF-1(3M) but not CP-DIF-1 induced phosphorylation (and therefore activation) of AMP kinase (AMPK) and promoted glucose consumption and metabolism. The DIF-induced glucose consumption was reduced by compound C (an AMPK inhibitor) or AMPK knock down. These data suggest that DIF-1 and DIF-1(3M) promote glucose uptake, at least in part, via an AMPK-dependent pathway in 3T3-L1 cells, whereas cellular metabolome analysis revealed that DIF-1 and DIF-1(3M) may act differently at least in part.

本文言語English
論文番号2293
ページ(範囲)1-13
ページ数13
ジャーナルInternational journal of molecular sciences
22
5
DOI
出版ステータスPublished - 2021 3 1
外部発表はい

ASJC Scopus subject areas

  • 触媒
  • 分子生物学
  • 分光学
  • コンピュータ サイエンスの応用
  • 物理化学および理論化学
  • 有機化学
  • 無機化学

フィンガープリント

「Dictyostelium differentiation-inducing factor-1 promotes glucose uptake, at least in part, via an ampk-dependent pathway in mouse 3t3-l1 cells」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル