Dielectric microsphere mediated transfection using a femtosecond laser

Mitsuhiro Terakawa, Yuto Tanaka

研究成果: Article

24 引用 (Scopus)

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We demonstrate the permeabilization of cell membranes by an enhanced optical field generated under polystyrene microspheres of 1000nm diameter excited by a femtosecond laser pulse. Fluorescent molecules and short interfering RNA (siRNA) have been successfully delivered to many cells in the irradiated area by a single 80 fs laser pulse at 800nm wavelength in the presence of antibody-conjugated polystyrene spheres. The ratios of the cells showing permeabilization were 38% and 21% for Fluorescein isothiocyanate-dextran and siRNA, respectively, at the laser fluence of 1:06 J=cm2. The present method has advantages both in high throughput of many cell treatments and precise processing of minute areas on cell membranes.

元の言語English
ページ(範囲)2877-2879
ページ数3
ジャーナルOptics Letters
36
発行部数15
DOI
出版物ステータスPublished - 2011 8 1

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ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics

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