Dielectric microsphere mediated transfection using a femtosecond laser

Mitsuhiro Terakawa, Yuto Tanaka

研究成果: Article

23 引用 (Scopus)

抄録

We demonstrate the permeabilization of cell membranes by an enhanced optical field generated under polystyrene microspheres of 1000nm diameter excited by a femtosecond laser pulse. Fluorescent molecules and short interfering RNA (siRNA) have been successfully delivered to many cells in the irradiated area by a single 80 fs laser pulse at 800nm wavelength in the presence of antibody-conjugated polystyrene spheres. The ratios of the cells showing permeabilization were 38% and 21% for Fluorescein isothiocyanate-dextran and siRNA, respectively, at the laser fluence of 1:06 J=cm2. The present method has advantages both in high throughput of many cell treatments and precise processing of minute areas on cell membranes.

元の言語English
ページ(範囲)2877-2879
ページ数3
ジャーナルOptics Letters
36
発行部数15
DOI
出版物ステータスPublished - 2011 8 1

Fingerprint

polystyrene
cells
lasers
dextrans
pulses
antibodies
fluence
wavelengths
molecules

ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics

これを引用

Dielectric microsphere mediated transfection using a femtosecond laser. / Terakawa, Mitsuhiro; Tanaka, Yuto.

:: Optics Letters, 巻 36, 番号 15, 01.08.2011, p. 2877-2879.

研究成果: Article

@article{8d7dc79b077046aa926b618598e733b3,
title = "Dielectric microsphere mediated transfection using a femtosecond laser",
abstract = "We demonstrate the permeabilization of cell membranes by an enhanced optical field generated under polystyrene microspheres of 1000nm diameter excited by a femtosecond laser pulse. Fluorescent molecules and short interfering RNA (siRNA) have been successfully delivered to many cells in the irradiated area by a single 80 fs laser pulse at 800nm wavelength in the presence of antibody-conjugated polystyrene spheres. The ratios of the cells showing permeabilization were 38{\%} and 21{\%} for Fluorescein isothiocyanate-dextran and siRNA, respectively, at the laser fluence of 1:06 J=cm2. The present method has advantages both in high throughput of many cell treatments and precise processing of minute areas on cell membranes.",
author = "Mitsuhiro Terakawa and Yuto Tanaka",
year = "2011",
month = "8",
day = "1",
doi = "10.1364/OL.36.002877",
language = "English",
volume = "36",
pages = "2877--2879",
journal = "Optics Letters",
issn = "0146-9592",
publisher = "The Optical Society",
number = "15",

}

TY - JOUR

T1 - Dielectric microsphere mediated transfection using a femtosecond laser

AU - Terakawa, Mitsuhiro

AU - Tanaka, Yuto

PY - 2011/8/1

Y1 - 2011/8/1

N2 - We demonstrate the permeabilization of cell membranes by an enhanced optical field generated under polystyrene microspheres of 1000nm diameter excited by a femtosecond laser pulse. Fluorescent molecules and short interfering RNA (siRNA) have been successfully delivered to many cells in the irradiated area by a single 80 fs laser pulse at 800nm wavelength in the presence of antibody-conjugated polystyrene spheres. The ratios of the cells showing permeabilization were 38% and 21% for Fluorescein isothiocyanate-dextran and siRNA, respectively, at the laser fluence of 1:06 J=cm2. The present method has advantages both in high throughput of many cell treatments and precise processing of minute areas on cell membranes.

AB - We demonstrate the permeabilization of cell membranes by an enhanced optical field generated under polystyrene microspheres of 1000nm diameter excited by a femtosecond laser pulse. Fluorescent molecules and short interfering RNA (siRNA) have been successfully delivered to many cells in the irradiated area by a single 80 fs laser pulse at 800nm wavelength in the presence of antibody-conjugated polystyrene spheres. The ratios of the cells showing permeabilization were 38% and 21% for Fluorescein isothiocyanate-dextran and siRNA, respectively, at the laser fluence of 1:06 J=cm2. The present method has advantages both in high throughput of many cell treatments and precise processing of minute areas on cell membranes.

UR - http://www.scopus.com/inward/record.url?scp=79961138416&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=79961138416&partnerID=8YFLogxK

U2 - 10.1364/OL.36.002877

DO - 10.1364/OL.36.002877

M3 - Article

C2 - 21808344

AN - SCOPUS:79961138416

VL - 36

SP - 2877

EP - 2879

JO - Optics Letters

JF - Optics Letters

SN - 0146-9592

IS - 15

ER -