Dielectrophoretic sorting system for screening of NAD(P)-dependent oxidoreductases.

Yuki Kanai, Shota Shimokihara, Shunya Shitara, Ryo Oyobiki, Takeshi Watanabe, Yasuaki Einaga, Kunihiko Wakamatsu, Yoshinori Matsumoto, Kei Fujiwara, Nobuhide Doi

研究成果: Conference contribution

抄録

Here we report the development of a flow injection analysis system based on electrochemical measurements and dielectrophoresis for screening of NAD(P)-dependent oxidoreductases. In this system, the activity of enzyme which is immobilized on microbeads together with its encoding DNA can be measured with a boron-doped diamond (BDD) electrode in each compartment (~30 nL) of the microfluidic system, and the large droplets (~30 nL) containing genes of enzymes with higher activity can be recovered by dielectrophoretic sorting.

本文言語English
ホスト出版物のタイトル21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017
出版社Chemical and Biological Microsystems Society
ページ1383-1384
ページ数2
ISBN(電子版)9780692941836
出版ステータスPublished - 2020
イベント21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017 - Savannah, United States
継続期間: 2017 10 222017 10 26

出版物シリーズ

名前21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017

Conference

Conference21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017
CountryUnited States
CitySavannah
Period17/10/2217/10/26

ASJC Scopus subject areas

  • Chemical Engineering (miscellaneous)
  • Bioengineering

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