Dietary lactoferrin alleviates age-related lacrimal gland dysfunction in mice

Motoko Kawashima, Tetsuya Kawakita, Takaaki Inaba, Naoko Okada, Masataka Ito, Shigeto Shimmura, Mitsuhiro Watanabe, Ken Shinmura, Kazuo Tsubota

研究成果: Article

30 引用 (Scopus)

抄録

Background: Decrease in lacrimal gland secretory function is related to age-induced dry eye disease. Lactoferrin, the main glycoprotein component of tears, has multiple functions, including anti-inflammatory effects and the promotion of cell growth. We investigated how oral administration of lactoferrin affects age-related lacrimal dysfunction. Methods and Findings: Twelve-month-old male C57BL/6Cr Slc mice were randomly divided into a control fed group and an oral lactoferrin treatment group. Tear function was measured at a 6-month time-point. After euthanasia, the lacrimal glands were subjected to histological examination with 8-hydroxy-2′-deoxyguanosine (8-OHdG) antibodies, and serum concentrations of 8-OHdG and hexanoyl-lysine adduct (HEL) were evaluated. Additionally, monocyte chemotactic protein-1(MCP-1) and tumor necrosis factor-α (TNF-α) gene expression levels were determined by real-time PCR. The volume of tear secretion was significantly larger in the treated group than in the control. Lactoferrin administration reduced inflammatory cell infiltration and the MCP-1 and TNF-α expression levels. Serum concentrations of 8-OHdG and HEL in the lactoferrin group were lower than those in the control group and were associated with attenuated 8-OHdG immunostaining of the lacrimal glands. Conclusion: Oral lactoferrin administration preserves lacrimal gland function in aged mice by attenuating oxidative damage and suppressing subsequent gland inflammation.

元の言語English
記事番号e33148
ジャーナルPLoS One
7
発行部数3
DOI
出版物ステータスPublished - 2012 3 27

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lacrimal apparatus
Lacrimal Apparatus
Lactoferrin
lactoferrin
Tears
mice
tumor necrosis factors
Chemokine CCL2
Control Groups
Lysine
Oral Administration
mouth
lysine
Tumor Necrosis Factor-alpha
eye diseases
Euthanasia
Eye Diseases
Cell growth
euthanasia
anti-inflammatory activity

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

これを引用

Dietary lactoferrin alleviates age-related lacrimal gland dysfunction in mice. / Kawashima, Motoko; Kawakita, Tetsuya; Inaba, Takaaki; Okada, Naoko; Ito, Masataka; Shimmura, Shigeto; Watanabe, Mitsuhiro; Shinmura, Ken; Tsubota, Kazuo.

:: PLoS One, 巻 7, 番号 3, e33148, 27.03.2012.

研究成果: Article

Kawashima, Motoko ; Kawakita, Tetsuya ; Inaba, Takaaki ; Okada, Naoko ; Ito, Masataka ; Shimmura, Shigeto ; Watanabe, Mitsuhiro ; Shinmura, Ken ; Tsubota, Kazuo. / Dietary lactoferrin alleviates age-related lacrimal gland dysfunction in mice. :: PLoS One. 2012 ; 巻 7, 番号 3.
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abstract = "Background: Decrease in lacrimal gland secretory function is related to age-induced dry eye disease. Lactoferrin, the main glycoprotein component of tears, has multiple functions, including anti-inflammatory effects and the promotion of cell growth. We investigated how oral administration of lactoferrin affects age-related lacrimal dysfunction. Methods and Findings: Twelve-month-old male C57BL/6Cr Slc mice were randomly divided into a control fed group and an oral lactoferrin treatment group. Tear function was measured at a 6-month time-point. After euthanasia, the lacrimal glands were subjected to histological examination with 8-hydroxy-2′-deoxyguanosine (8-OHdG) antibodies, and serum concentrations of 8-OHdG and hexanoyl-lysine adduct (HEL) were evaluated. Additionally, monocyte chemotactic protein-1(MCP-1) and tumor necrosis factor-α (TNF-α) gene expression levels were determined by real-time PCR. The volume of tear secretion was significantly larger in the treated group than in the control. Lactoferrin administration reduced inflammatory cell infiltration and the MCP-1 and TNF-α expression levels. Serum concentrations of 8-OHdG and HEL in the lactoferrin group were lower than those in the control group and were associated with attenuated 8-OHdG immunostaining of the lacrimal glands. Conclusion: Oral lactoferrin administration preserves lacrimal gland function in aged mice by attenuating oxidative damage and suppressing subsequent gland inflammation.",
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