Differences in integrin-dependent phagocytosis among three hemocyte subpopulations of the Pacific oyster "Crassostrea gigas"

Kazutaka Terahara, Keisuke G. Takahashi, Akifumi Nakamura, Makoto Osada, Masaki Yoda, Takachika Hiroi, Masatomo Hirasawa, Katsuyoshi Mori

研究成果: Article査読

54 被引用数 (Scopus)

抄録

Integrins play a key role in immunoresponses such as attachment, spreading, and phagocytosis in invertebrate hemocytes. This study was designed to identify integrin expression patterns at the hemocyte subpopulation level, and correlate the expression levels with phagocytic ability. First, we cloned a β integrin from Crassostrea gigas hemocytes and used real-time RT-PCR to analyze the quantitative expression level of its encoding mRNA. The expression level in hyalinocytes was significantly higher than that in granulocytes and agranulocytes. Subsequently, we investigated the phagocytic ability of each subpopulation using anti-α5β1 integrin antibody, and found that phagocytosis of hyalinocytes was inhibited by neutralization with the antibody but enhanced against the antibody-conjugated microspheres. In contrast, phagocytic abilities of granulocytes and agranulocytes showed high and zero levels, respectively, regardless of the antibody. These results suggest that phagocytosis of hyalinocytes is regulated by an integrin-dependent mechanism and that of granulocytes is elicited by other functional receptors.

本文言語English
ページ(範囲)667-683
ページ数17
ジャーナルDevelopmental and Comparative Immunology
30
8
DOI
出版ステータスPublished - 2006

ASJC Scopus subject areas

  • 免疫学
  • 発生生物学

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