Differential adaptive responses to 1- or 2-day fasting in various mouse tissues revealed by quantitative PCR analysis

Junya Yamamoto, Shotaro Kamata, Asumi Miura, Tomoko Nagata, Ryo Kainuma, Isao Ishii

研究成果: Article査読

17 被引用数 (Scopus)

抄録

Dietary or caloric restriction confers various clinical benefits. Short-term fasting of mice is a common experimental procedure that may involve systemic metabolic remodeling, which may significantly affect experimental outputs. This study evaluated adaptive cellular responses after 1- or 2-day fasting in 13 mouse tissues by quantitative PCR using 15 marker primer sets for the activation of ubiquitin-proteasome (. Atrogin-1 and MuRF1), autophagy-lysosome (. LC3b, p62 and Lamp2), amino acid response (. Asns, Trib3, Herpud1, xCT, and Chop), Nrf2-mediated antioxidant (. HO-1 and Gsta1), and amino acid transport (. Slc38a2, Slc7a5, and Slc7a1) systems. Differential activation profiles obtained in seven highly (thymus, liver, spleen, and small intestine) or mildly (stomach, kidney, and colon) atrophied tissues as well as in six non-atrophied tissues (brain, eye, lung, heart, skeletal muscle, and testis) suggested tissue-specific active metabolic remodeling.

本文言語English
ページ(範囲)357-368
ページ数12
ジャーナルFEBS Open Bio
5
DOI
出版ステータスPublished - 2015 4月 21

ASJC Scopus subject areas

  • 生化学、遺伝学、分子生物学(全般)

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