TY - JOUR
T1 - Differential blocking effects of a spider toxin on synaptic and glutamate responses in the afferent synapse of the acoustico-lateralis receptors of Plotosus
AU - Nagai, Takatoshi
AU - Obara, Shosaku
AU - Kawai, Nobufumi
N1 - Funding Information:
1 Abe, T., Kawai, N. and Miwa, A., Effectso f a spidert oxin on the glutaminergsicy napseo f lobsterm uscleJ,. Physiol. (Lond.), 339( 1983)2 43--252. 2 BledsoeJ,r ., S. C. and Bobbin,R . P., Effe~ctosf D-a-ami-noadipatoen excitatioonf afferenfti bersi n the lateralli ne of Xenopus laevis, Neurosci. Lett., 32 (1982)315-320. 3 Bobbin,R . P., Glutamataen da spartatme imict heafferent transmitteirn the cochlea,E xp. Brain Res., 34 (1979) 38%393. 4 Curtis,D . R. andJ ohnstonG, . A. R., Amino acidt ransmit-ters in the mammalianc entraln ervouss ystem,E rgebn. Physiol., 69 (1974)9 7-188. 5 Fex, J. and Martin,M . R., Lack of effei:to f oL-a-aminoadi-pate,a n excitatoraym inoa cida ntagonisto,n cat auditory nerver esponsetso sound,N europharmacology, 19 (1980) 80%811. 6 KawagoeR, ., OnoderaK, . and TakeuchiA, ., Releaseo f glutamatefr om the crayfishn euromusculajur nction,J . Physiol. (Lond.), 312( 1981)225-236. 7 Kawai, N., Niwa, A. and Abe, T., Spiderv enomc ontains specificr eceptobr lockero f glutaminergsicy napsesB, rain Research, 247( 1982)1 69-171. 8 Kawai, N., Yamagishi,S ., Saito, M. and Furuya, K., Blockadeo f synaptict ransmissioinn the squid giant syn-apseb y a spidert oxin,B rain Research, 278( 1983)3 46-349. 9 Klinke, R. and Oertel,W ., Amino acids-- putativea ffer-ent transmitteinr the cochlea?E xp. Brain Res., 30 (1977) 145-148. 10 Medina,J . E. and DrescherD, . G., The amino-acidco n-tento f perilympha ndc erebrospinfallu id from guinea-pigs and the effecto f noise on the amino-acidc ompositioonf perilymphN,e uroscience, 6 ( 1981)5 05-509. 11 Nagai,T . andO bara,S ., Iontophoretaicp plicatioonf acidic amino acids in ampullarye lectroreceptoorsf Plotosus, This work was supportedin part by the grantsf rom the Ministry of Education,S ciencea nd Culture of Japan, No. 548101,5 7770115a nd 58370005.
PY - 1984/5/21
Y1 - 1984/5/21
N2 - The hypothesis that glutamate is the afferent transmitter in the acoustico-lateralis receptors was examined in Plotosus electroreceptors. JSTX, a spider toxin known to specifically block glutamate receptors, irreversibly abolished afferent impulse discharges induced by iontophoretically applied glutamate, whereas those induced synaptically by focal stimulation of receptor cells were little affected. Such differential blocking effects by JSTX, complementary to other biochemical data, further provide pharmacological evidence against the glutamate hypothesis.
AB - The hypothesis that glutamate is the afferent transmitter in the acoustico-lateralis receptors was examined in Plotosus electroreceptors. JSTX, a spider toxin known to specifically block glutamate receptors, irreversibly abolished afferent impulse discharges induced by iontophoretically applied glutamate, whereas those induced synaptically by focal stimulation of receptor cells were little affected. Such differential blocking effects by JSTX, complementary to other biochemical data, further provide pharmacological evidence against the glutamate hypothesis.
KW - acoustico-lateralis receptor
KW - afferent synapse
KW - glutamate receptor
KW - iontophoresis
KW - neurotransmitter
KW - spider toxin
UR - http://www.scopus.com/inward/record.url?scp=0021282077&partnerID=8YFLogxK
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U2 - 10.1016/0006-8993(84)91357-X
DO - 10.1016/0006-8993(84)91357-X
M3 - Article
C2 - 6145500
AN - SCOPUS:0021282077
VL - 300
SP - 183
EP - 187
JO - Molecular Brain Research
JF - Molecular Brain Research
SN - 0006-8993
IS - 1
ER -