Differential effects of desmoglein 1 and desmoglein 3 on desmosome formation

Yasushi Hanakawa, Masayuki Amagai, Yuji Shirakata, Yoko Yahata, Sho Tokumaru, Kenshi Yamasaki, Mikiko Tohyama, Koji Sayama, Koji Hashimoto

研究成果: Article

19 引用 (Scopus)

抄録

The desmoglein plays an important part in the formation of desmosomes. We constructed recombinant adenoviruses containing desmoglein 1 and desmoglein 3 derivatives partly lacking the extracellular domain (desmoglein 1ΔEC and desmoglein 3ΔEC, respectively), and full-length desmoglein 1 and desmoglein 3 and studied the involvement of desmoglein 1 and desmoglein 3 in desmosome formation. During low-level expression of desmoglein 3DEC in transduced HaCaT cells, keratin insertion at cell-cell contact sites was only partially inhibited and desmoplakin was partially stained at cell-cell contact sites. Low-level expression of desmoglein 1DEC, however, resulted in complete inhibition of keratin insertion at the cell-cell contact sites, and desmoplakin was stained in perinuclear dots. These results indicate the dominant-negative effect of desmoglein 1ΔEC on desmosome formation was stronger than that of desmoglein 3ΔEC. Desmoglein 1ΔEC coprecipitated plakoglobin to approximately the same extent as desmoglein 3ΔEC. Therefore, we conclude that the dominant-negative effect of desmoglein 1ΔEC is not simply due to plakoglobin sequestration. On the other hand, during low-level expression of full-length desmoglein 3 and desmoglein 1, they both colocalized with desmoplakin. During high-level expression, however, keratin insertion at cell-cell contact sites was inhibited in desmoglein 1 but not in desmoglein 3, and desmoplakin was stained at cell-cell contact sites in desmoglein 3 but not in desmoglein 1. These data suggest desmoglein 1 and desmoglein 3 expressed at low level were incorporated into desmosome but at high-level expression, desmoglein 1 disrupted desmosomes but desmoglein 3 did not. Our findings provide biologic evidence that desmoglein 1 and desmoglein 3 play a different functional role in cell-cell adhesion of keratinocytes.

元の言語English
ページ(範囲)1231-1236
ページ数6
ジャーナルJournal of Investigative Dermatology
119
発行部数6
DOI
出版物ステータスPublished - 2002

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Desmogleins
Desmoglein 3
Desmoglein 1
Desmosomes
Desmoplakins
Keratins
gamma Catenin
Cell adhesion
Keratinocytes
Adenoviridae
Cell Adhesion

ASJC Scopus subject areas

  • Dermatology

これを引用

Hanakawa, Y., Amagai, M., Shirakata, Y., Yahata, Y., Tokumaru, S., Yamasaki, K., ... Hashimoto, K. (2002). Differential effects of desmoglein 1 and desmoglein 3 on desmosome formation. Journal of Investigative Dermatology, 119(6), 1231-1236. https://doi.org/10.1046/j.1523-1747.2002.19648.x

Differential effects of desmoglein 1 and desmoglein 3 on desmosome formation. / Hanakawa, Yasushi; Amagai, Masayuki; Shirakata, Yuji; Yahata, Yoko; Tokumaru, Sho; Yamasaki, Kenshi; Tohyama, Mikiko; Sayama, Koji; Hashimoto, Koji.

:: Journal of Investigative Dermatology, 巻 119, 番号 6, 2002, p. 1231-1236.

研究成果: Article

Hanakawa, Y, Amagai, M, Shirakata, Y, Yahata, Y, Tokumaru, S, Yamasaki, K, Tohyama, M, Sayama, K & Hashimoto, K 2002, 'Differential effects of desmoglein 1 and desmoglein 3 on desmosome formation', Journal of Investigative Dermatology, 巻. 119, 番号 6, pp. 1231-1236. https://doi.org/10.1046/j.1523-1747.2002.19648.x
Hanakawa, Yasushi ; Amagai, Masayuki ; Shirakata, Yuji ; Yahata, Yoko ; Tokumaru, Sho ; Yamasaki, Kenshi ; Tohyama, Mikiko ; Sayama, Koji ; Hashimoto, Koji. / Differential effects of desmoglein 1 and desmoglein 3 on desmosome formation. :: Journal of Investigative Dermatology. 2002 ; 巻 119, 番号 6. pp. 1231-1236.
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AU - Hanakawa, Yasushi

AU - Amagai, Masayuki

AU - Shirakata, Yuji

AU - Yahata, Yoko

AU - Tokumaru, Sho

AU - Yamasaki, Kenshi

AU - Tohyama, Mikiko

AU - Sayama, Koji

AU - Hashimoto, Koji

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N2 - The desmoglein plays an important part in the formation of desmosomes. We constructed recombinant adenoviruses containing desmoglein 1 and desmoglein 3 derivatives partly lacking the extracellular domain (desmoglein 1ΔEC and desmoglein 3ΔEC, respectively), and full-length desmoglein 1 and desmoglein 3 and studied the involvement of desmoglein 1 and desmoglein 3 in desmosome formation. During low-level expression of desmoglein 3DEC in transduced HaCaT cells, keratin insertion at cell-cell contact sites was only partially inhibited and desmoplakin was partially stained at cell-cell contact sites. Low-level expression of desmoglein 1DEC, however, resulted in complete inhibition of keratin insertion at the cell-cell contact sites, and desmoplakin was stained in perinuclear dots. These results indicate the dominant-negative effect of desmoglein 1ΔEC on desmosome formation was stronger than that of desmoglein 3ΔEC. Desmoglein 1ΔEC coprecipitated plakoglobin to approximately the same extent as desmoglein 3ΔEC. Therefore, we conclude that the dominant-negative effect of desmoglein 1ΔEC is not simply due to plakoglobin sequestration. On the other hand, during low-level expression of full-length desmoglein 3 and desmoglein 1, they both colocalized with desmoplakin. During high-level expression, however, keratin insertion at cell-cell contact sites was inhibited in desmoglein 1 but not in desmoglein 3, and desmoplakin was stained at cell-cell contact sites in desmoglein 3 but not in desmoglein 1. These data suggest desmoglein 1 and desmoglein 3 expressed at low level were incorporated into desmosome but at high-level expression, desmoglein 1 disrupted desmosomes but desmoglein 3 did not. Our findings provide biologic evidence that desmoglein 1 and desmoglein 3 play a different functional role in cell-cell adhesion of keratinocytes.

AB - The desmoglein plays an important part in the formation of desmosomes. We constructed recombinant adenoviruses containing desmoglein 1 and desmoglein 3 derivatives partly lacking the extracellular domain (desmoglein 1ΔEC and desmoglein 3ΔEC, respectively), and full-length desmoglein 1 and desmoglein 3 and studied the involvement of desmoglein 1 and desmoglein 3 in desmosome formation. During low-level expression of desmoglein 3DEC in transduced HaCaT cells, keratin insertion at cell-cell contact sites was only partially inhibited and desmoplakin was partially stained at cell-cell contact sites. Low-level expression of desmoglein 1DEC, however, resulted in complete inhibition of keratin insertion at the cell-cell contact sites, and desmoplakin was stained in perinuclear dots. These results indicate the dominant-negative effect of desmoglein 1ΔEC on desmosome formation was stronger than that of desmoglein 3ΔEC. Desmoglein 1ΔEC coprecipitated plakoglobin to approximately the same extent as desmoglein 3ΔEC. Therefore, we conclude that the dominant-negative effect of desmoglein 1ΔEC is not simply due to plakoglobin sequestration. On the other hand, during low-level expression of full-length desmoglein 3 and desmoglein 1, they both colocalized with desmoplakin. During high-level expression, however, keratin insertion at cell-cell contact sites was inhibited in desmoglein 1 but not in desmoglein 3, and desmoplakin was stained at cell-cell contact sites in desmoglein 3 but not in desmoglein 1. These data suggest desmoglein 1 and desmoglein 3 expressed at low level were incorporated into desmosome but at high-level expression, desmoglein 1 disrupted desmosomes but desmoglein 3 did not. Our findings provide biologic evidence that desmoglein 1 and desmoglein 3 play a different functional role in cell-cell adhesion of keratinocytes.

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