Direct influences of pro-inflammatory cytokines (IL-1β, TNF-α, IL-6) on the proliferation and cell-surface antigen expression of cancer cells

Shinji Kuninaka, Tokujiro Yano, Hideki Yokoyama, Yasuro Fukuyama, Yasuhiro Terazaki, Tadashi Uehara, Takanori Kanematsu, Hiroshi Asoh, Yukito Ichinose

研究成果: Article査読

47 被引用数 (Scopus)

抄録

Pro-inflammatory cytokines, e.g. interleukin 1 (IL-1), tumour necrosis factor α (TNF-α), IL-6 produced by surgical intervention or non-specific immunotherapy may directly affect both the growth and the metastasis of tumour cells. It is therefore important to clarify the direct influence of pro-inflammatory cytokines on tumour cells in order to obtain a better knowledge of anti-tumour therapy. Four human lung cancer cell lines were used. The tumour cells were incubated for 72 h in the presence of various concentrations of IL-1β, TNF-α, or IL-6 and then the proliferative response was assessed by an MTT assay. After 14 days of culture with each pro-inflammatory cytokine, the cell-surface antigen expressions (HLA-class I, HLA-class II, CEA, sialyl Lewis(x)) were assessed by an immunocytochemical staining method. Among the various combinations of tumour cells (PC-9, PC-12, QG-56, QG-95) and cytokines IL-1β, TNF-α, IL-6), only TNF-α significantly exhibited an antiproliferative effect against PC-9 cells. However, various modulations of the cell-surface antigen expression by the cytokines were observed. The HLA-class I antigen expression of PC-9 was augmented by either TNF-α or IL-1β. Furthermore, IL-1β was able to induce CEA in PC-9, QG-56, and QG-95 cells while TNF-α was able to enhance the expression of sialyl Lewis(x) in QG-95 cells. Although the influence of pro-inflammatory cytokines on the growth of tumour cells was only slight, some modulations of the cell-surface antigen expression were notable. The augmentation of HLA-class I expression can thus improve the immunogenicity of tumour cells while the induction of CEA or sialyl Lewis(x) may therefore be associated with the promotion of metastasis.

本文言語English
ページ(範囲)8-11
ページ数4
ジャーナルCytokine
12
1
DOI
出版ステータスPublished - 2000 1月
外部発表はい

ASJC Scopus subject areas

  • 免疫アレルギー学
  • 免疫学
  • 生化学
  • 分子生物学
  • 血液学

フィンガープリント

「Direct influences of pro-inflammatory cytokines (IL-1β, TNF-α, IL-6) on the proliferation and cell-surface antigen expression of cancer cells」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル