Direct profiling of the phospholipid composition of adult Caenorhabditis elegans using whole-body imaging mass spectrometry

Saira Hameed, Koji Ikegami, Eiji Sugiyama, Shoko Matsushita, Yoshishige Kimura, Takahiro Hayasaka, Yuki Sugiura, Noritaka Masaki, Michihiko Waki, Isao Ohta, Md Amir Hossen, Mitsutoshi Setou

研究成果: Article査読

6 被引用数 (Scopus)

抄録

A protocol for the direct analysis of the phospholipid composition in the whole body of adult soil nematode, Caenorhabditis elegans (C. elegans), was developed, which combined freeze-cracking of the exoskeletal cuticle and matrix-assisted laser desorption/ionization-imaging mass spectrometry (MALDI-IMS). Biomolecules in the m/z range from 700 to 900 were more effectively detected in the freezecracked than from simple frozen adult nematode bodies. Different distribution of biomolecules was observed in a nematode body when the matrix was applied with a sublimation deposition method. The whole-body IMS technique was applied on genetically deficient mutant C. elegans to combine whole-body lipidomics and genetics, by comparing the fatty acid compositions, especially of the phosphatidylcholine (PC) species, between the wild-type and fat-1 mutants, which lack the gene encoding an n-3 fatty acid desaturase. A significant reduction of PC(20:5/20:5) and PC(20:4/20:5) and a marked increase of PC(20:4/20:4), PC(20:3/20:4), and PC(20:3/20:3) were detected in the fat-1 mutants in positive ion mode. In addition, phospholipid compositions other than PCs were analyzed in negative ion mode. A loss of a possible phosphatidylinositol (PI) with 18:0/ 20:5 and a compensative accumulation of putative PI(18:0/20:4) were detected in the fat-1 mutants. In conclusion, the whole-body MALDI-IMS technique is useful for the profiling of multiple biomolecules in C. elegans in both intra-and inter-individual levels.

本文言語English
ページ(範囲)7589-7602
ページ数14
ジャーナルAnalytical and bioanalytical chemistry
407
25
DOI
出版ステータスPublished - 2017 10

ASJC Scopus subject areas

  • 分析化学
  • 生化学

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