TY - JOUR
T1 - Direct visualization of general anesthetic propofol on neurons by stimulated Raman scattering microscopy
AU - Oda, Robert
AU - Shou, Jingwen
AU - Zhong, Wenying
AU - Ozeki, Yasuyuki
AU - Yasui, Masato
AU - Nuriya, Mutsuo
N1 - Funding Information:
We would like to thank Dr. Yoichiro Abe for providing HeLa cells and Olympus Corporation for technical assistance. Funding was provided by Japan Science and Technology Agency Precursory Research for Embryonic Science and Technology (JST PRESTO) ( JPMJPR17G6 ), Japan Science and Technology Agency Core Research for Evolutional Science and Technology (JST CREST) ( JPMJCR1872 ), and Japan Society for the Promotion of Science Grant-in-Aid for Scientific Research (JSPS KAKENHI) ( 20K20593 , 20H02881 ).
Publisher Copyright:
© 2022 The Authors
PY - 2022/3/18
Y1 - 2022/3/18
N2 - The consensus for the precise mechanism of action of general anesthetics is through allosteric interactions with GABA receptors in neurons. However, it has been speculated that these anesthetics may also interact with the plasma membrane on some level. Owing to the small size of anesthetics, direct visualization of these interactions is difficult to achieve. We demonstrate the ability to directly visualize a deuterated analog of propofol in living cells using stimulated Raman scattering (SRS) microscopy. Our findings support the theory that propofol is highly concentrated and interacts primarily through non-specific binding to the plasma membrane of neurons. Additionally, we show that SRS microscopy can be used to monitor the dynamics of propofol binding using real-time, live-cell imaging.
AB - The consensus for the precise mechanism of action of general anesthetics is through allosteric interactions with GABA receptors in neurons. However, it has been speculated that these anesthetics may also interact with the plasma membrane on some level. Owing to the small size of anesthetics, direct visualization of these interactions is difficult to achieve. We demonstrate the ability to directly visualize a deuterated analog of propofol in living cells using stimulated Raman scattering (SRS) microscopy. Our findings support the theory that propofol is highly concentrated and interacts primarily through non-specific binding to the plasma membrane of neurons. Additionally, we show that SRS microscopy can be used to monitor the dynamics of propofol binding using real-time, live-cell imaging.
KW - Cell biology
KW - Cellular neuroscience
KW - Techniques in neuroscience
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U2 - 10.1016/j.isci.2022.103936
DO - 10.1016/j.isci.2022.103936
M3 - Article
AN - SCOPUS:85125501171
SN - 2589-0042
VL - 25
JO - iScience
JF - iScience
IS - 3
M1 - 103936
ER -