Direct visualization of general anesthetic propofol on neurons by stimulated Raman scattering microscopy

Robert Oda, Jingwen Shou, Wenying Zhong, Yasuyuki Ozeki, Masato Yasui, Mutsuo Nuriya

研究成果: Article査読

抄録

The consensus for the precise mechanism of action of general anesthetics is through allosteric interactions with GABA receptors in neurons. However, it has been speculated that these anesthetics may also interact with the plasma membrane on some level. Owing to the small size of anesthetics, direct visualization of these interactions is difficult to achieve. We demonstrate the ability to directly visualize a deuterated analog of propofol in living cells using stimulated Raman scattering (SRS) microscopy. Our findings support the theory that propofol is highly concentrated and interacts primarily through non-specific binding to the plasma membrane of neurons. Additionally, we show that SRS microscopy can be used to monitor the dynamics of propofol binding using real-time, live-cell imaging. The strategy used to visualize propofol can be applied to other small molecule drugs that have been previously invisible to traditional imaging techniques

本文言語English
論文番号103936
ジャーナルiScience
25
3
DOI
出版ステータスPublished - 2022 3月 18

ASJC Scopus subject areas

  • 一般

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